Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-9-6
pubmed:abstractText
Mammalian myeloid and epithelial cells express several kinds of antibacterial peptides (alpha-/beta-defensins and cathelicidins) that contribute to the innate host defense by killing invading micro-organisms. In this study we evaluated the LPS-neutralizing activities of cathelicidin peptides human CAP18 (cationic antibacterial proteins of 18 kDa) and guinea pig CAP11 using the CD14(+) murine macrophage cell line RAW264.7 and the murine endotoxin shock model. Flow cytometric analysis revealed that CAP18 and CAP11 inhibited the binding of FITC-conjugated LPS to RAW264.7 cells. Likewise, Northern and Western blot analyses indicated that CAP18 and CAP11 suppressed LPS-induced TNF-alpha mRNA and protein expression by RAW264.7 cells. Interestingly, CAP18 and CAP11 possessed LPS-binding activities, and they strongly suppressed the interaction of LPS with LPS binding protein that mediates the transport of LPS to CD14 to facilitate the activation of CD14(+) cells by LPS. Moreover, when CAP18 and CAP11 were preincubated with RAW264.7 cells, they bound to the cell surface CD14 and inhibited the binding of FITC-LPS to the cells. Furthermore, in the murine endotoxin shock model, CAP18 or CAP11 administration inhibited the binding of LPS to CD14(+) cells (peritoneal macrophages) and suppressed LPS-induced TNF-alpha expression by these cells. Together these observations indicate that cathelicidin peptides CAP18 and CAP11 probably exert protective actions against endotoxin shock by blocking the binding of LPS to CD14(+) cells, thereby suppressing the production of cytokines by these cells via their potent binding activities for LPS and CD14.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Acute-Phase Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14, http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides, http://linkedlifedata.com/resource/pubmed/chemical/CAP18 lipopolysaccharide-binding..., http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cathelicidins, http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Prodrugs, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha, http://linkedlifedata.com/resource/pubmed/chemical/cathelicidin antimicrobial peptide, http://linkedlifedata.com/resource/pubmed/chemical/lipopolysaccharide-binding protein
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3329-38
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11544322-Acute-Phase Proteins, pubmed-meshheading:11544322-Amino Acid Sequence, pubmed-meshheading:11544322-Animals, pubmed-meshheading:11544322-Anti-Bacterial Agents, pubmed-meshheading:11544322-Antigens, CD14, pubmed-meshheading:11544322-Antimicrobial Cationic Peptides, pubmed-meshheading:11544322-Carrier Proteins, pubmed-meshheading:11544322-Cathelicidins, pubmed-meshheading:11544322-Cell Line, pubmed-meshheading:11544322-Depression, Chemical, pubmed-meshheading:11544322-Drug Evaluation, Preclinical, pubmed-meshheading:11544322-Gene Expression Regulation, pubmed-meshheading:11544322-Guinea Pigs, pubmed-meshheading:11544322-Humans, pubmed-meshheading:11544322-Lipopolysaccharides, pubmed-meshheading:11544322-Macrophages, pubmed-meshheading:11544322-Macrophages, Peritoneal, pubmed-meshheading:11544322-Male, pubmed-meshheading:11544322-Membrane Glycoproteins, pubmed-meshheading:11544322-Mice, pubmed-meshheading:11544322-Mice, Inbred C57BL, pubmed-meshheading:11544322-Molecular Sequence Data, pubmed-meshheading:11544322-Prodrugs, pubmed-meshheading:11544322-Protein Binding, pubmed-meshheading:11544322-RNA, Messenger, pubmed-meshheading:11544322-Shock, Septic, pubmed-meshheading:11544322-Tumor Necrosis Factor-alpha
pubmed:year
2001
pubmed:articleTitle
Cathelicidin family of antibacterial peptides CAP18 and CAP11 inhibit the expression of TNF-alpha by blocking the binding of LPS to CD14(+) cells.
pubmed:affiliation
Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan. nagaokai@med.juntendo.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't