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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
2001-9-5
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pubmed:abstractText |
The feasibility of using oligodeoxynucleotides (ODN) containing unmethylated CpG motifs as parenteral adjuvants for subunit vaccines against RSV was tested in BALB/c mice. Compared with immunization with natural F protein adsorbed to aluminum hydroxide (F/AlOH) adjuvant alone, coadministration of F/AlOH with CpG ODN resulted in statistically significant increases in serum neutralization titers, an enhanced generation of splenic antigen-dependent killer cell precursors, and accelerated clearance of infectious virus from lungs 4 days after challenge. The statistically significant increases in serum IFNgamma and anti-F protein IgG2a titers, and significantly diminished pulmonary IL-5 and eosinophilia after challenge indicated that CpG ODN enhanced the ability of F/AlOH to elicit type 1 immune responses. F protein-specific serum IgE titers were also reduced. Further analysis of pulmonary inflammatory cells demonstrated an expansion of CD8(+) T cells, relative to the CD4(+) T cell compartment. The potency of CpG ODN was not adversely affected in gene knockout mice devoid of the p35 chain of the IL-12 heterodimer. Taken together, the results suggest a novel formulation for naïve recipients of F protein-based subunit vaccines that does not result in a type 2 phenotype.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Respiratory Syncytial Virus Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4874-82
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11535341-Adjuvants, Immunologic,
pubmed-meshheading:11535341-Animals,
pubmed-meshheading:11535341-Antibodies, Viral,
pubmed-meshheading:11535341-Antibody Specificity,
pubmed-meshheading:11535341-CpG Islands,
pubmed-meshheading:11535341-Dimerization,
pubmed-meshheading:11535341-Female,
pubmed-meshheading:11535341-Immunity, Cellular,
pubmed-meshheading:11535341-Immunoglobulin G,
pubmed-meshheading:11535341-Injections, Intramuscular,
pubmed-meshheading:11535341-Interferon-gamma,
pubmed-meshheading:11535341-Interleukin-12,
pubmed-meshheading:11535341-Interleukin-5,
pubmed-meshheading:11535341-Killer Cells, Natural,
pubmed-meshheading:11535341-Lung,
pubmed-meshheading:11535341-Methylation,
pubmed-meshheading:11535341-Mice,
pubmed-meshheading:11535341-Mice, Inbred BALB C,
pubmed-meshheading:11535341-Mice, Knockout,
pubmed-meshheading:11535341-Pneumonia, Viral,
pubmed-meshheading:11535341-Protein Subunits,
pubmed-meshheading:11535341-Pulmonary Eosinophilia,
pubmed-meshheading:11535341-Rats,
pubmed-meshheading:11535341-Rats, Sprague-Dawley,
pubmed-meshheading:11535341-Respiratory Syncytial Virus Infections,
pubmed-meshheading:11535341-Respiratory Syncytial Virus Vaccines,
pubmed-meshheading:11535341-Respiratory Syncytial Viruses,
pubmed-meshheading:11535341-Spleen,
pubmed-meshheading:11535341-Vaccination,
pubmed-meshheading:11535341-Viral Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
CpG containing oligodeoxynucleotides are potent adjuvants for parenteral vaccination with the fusion (F) protein of respiratory syncytial virus (RSV).
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pubmed:affiliation |
Department of Immunology Research, Wyeth-Lederle Vaccines, 211 Bailey Road, West Henrietta, NY 14586, USA. hancocg@war.wyeth.com
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pubmed:publicationType |
Journal Article
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