Linked Life Data

11527802

Source:http://linkedlifedata.com/resource/pubmed/id/11527802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-8-30
pubmed:abstractText
A new rapid direct immunofluorescence assay, the SimulFluor direct fluorescent-antibody (DFA) assay, which can simultaneously detect herpes simplex virus types 1 and 2 (HSV-1 and -2) and varicella-zoster virus (VZV), was evaluated in comparison with our current standard procedures of (i) shell vial direct immunoperoxidase (shell vial IP) staining and cell culture for detection of HSV and (ii) cytospin DFA staining for VZV detection. A total of 517 vesicular, oral, genital, and skin lesion specimens were tested by all three procedures. For HSV detection, the SimulFluor DFA assay had an overall sensitivity, specificity, positive predictive value, and negative predictive value of 80.0, 98.3, 92.3, and 95.1%, respectively, when compared to culture. Shell vial IP staining had a sensitivity, specificity, positive predictive value, and negative predictive value of 87.6, 100, 100, and 96.9%, respectively, when compared with cell culture. The SimulFluor DFA assay, however, offers same-day, 1.5-hours results versus a 1- to 2-day wait for shell vial IP staining results and a 1- to 6-day wait for culture results for HSV. For VZV detection SimulFluor DFA staining detected 27 positive specimens as compared to 31 by our standard cytospin DFA technique--a correlation of 87.1%. A positive SimulFluor reaction for VZV is indicated by yellow-gold fluorescence compared to the bright apple-green fluorescence observed by cytospin DFA staining. There is no difference in turnaround time between the two assays. The SimulFluor DFA assay is a rapid immunofluorescence assay that can detect 80% of the HSV-positive specimens and 87% of the VZV-positive specimens with a 1.5-h turnaround time.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
909-12
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11527802-Animals, pubmed-meshheading:11527802-Cell Culture Techniques, pubmed-meshheading:11527802-Cell Line, pubmed-meshheading:11527802-Centrifugation, pubmed-meshheading:11527802-Cercopithecus aethiops, pubmed-meshheading:11527802-Cytopathogenic Effect, Viral, pubmed-meshheading:11527802-Female, pubmed-meshheading:11527802-Fluorescent Antibody Technique, Direct, pubmed-meshheading:11527802-Herpes Simplex, pubmed-meshheading:11527802-Herpesvirus 3, Human, pubmed-meshheading:11527802-Humans, pubmed-meshheading:11527802-Immunoenzyme Techniques, pubmed-meshheading:11527802-Male, pubmed-meshheading:11527802-Predictive Value of Tests, pubmed-meshheading:11527802-Sensitivity and Specificity, pubmed-meshheading:11527802-Simplexvirus, pubmed-meshheading:11527802-Staining and Labeling, pubmed-meshheading:11527802-Vero Cells, pubmed-meshheading:11527802-Virus Cultivation
pubmed:year
2001
pubmed:articleTitle
Comparison of Chemicon SimulFluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence staining for detection of varicella-zoster virus.
pubmed:affiliation
Virology Section, Clinical Microbiology Department, Provincial Laboratory, Regina, Saskatchewan S4S 5W6, Canada. echan@health.gov.sk.ca
pubmed:publicationType
Journal Article, Comparative Study