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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-8-28
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pubmed:abstractText |
Thyroid glands affected by Graves' disease (GD) show striking leukocytic infiltration, mainly by T-cells. The mechanisms by which the various leukocytes are maintained in the thyroid are unknown. Growth-regulated oncogene-alpha (GRO-alpha) in interaction with its receptor CXCR2 is a chemoattractant for both T-cells and neutrophils and may be one of the chemokines involved in the cell maintenance. GRO-alpha and CD18 mRNA as a marker of leukocytic infiltration were quantified in thyroid tissue using competitive RT-PCR. We found very high GRO-alpha mRNA levels in all thyroid tissues. In GD patients (n=16), the GRO-alpha mRNA did not correlate with the CD18 mRNA level or thyroid peroxidase and TSH-receptor antibodies in patients' sera. In thyroid autonomy (n=10), the GRO-alpha mRNA levels were significantly lower in autonomous single adenomas compared with the corresponding normal tissue. In order to define the cellular source of GRO-alpha mRNA and protein, we examined various thyroid-derived cells. Thyrocytes, thyroid-derived leukocytes and fibroblasts showed basal GRO-alpha mRNA and protein expression, which was remarkably upregulated by different stimuli in vitro. The expression of GRO-alpha by thyroid carcinoma cell lines confirms that thyrocytes may actually produce GRO-alpha. As shown by flow cytometry and immunohistology, CD68+ monocytes/macrophages are the only cell population strongly expressing CXCR2 in the thyroid.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8B
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-0795
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
513-20
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11524231-Adenoma,
pubmed-meshheading:11524231-Adult,
pubmed-meshheading:11524231-Cell Culture Techniques,
pubmed-meshheading:11524231-Chemokine CXCL1,
pubmed-meshheading:11524231-Chemokines, CXC,
pubmed-meshheading:11524231-Chemotactic Factors,
pubmed-meshheading:11524231-Female,
pubmed-meshheading:11524231-Gene Expression Regulation,
pubmed-meshheading:11524231-Graves Disease,
pubmed-meshheading:11524231-Growth Substances,
pubmed-meshheading:11524231-Humans,
pubmed-meshheading:11524231-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11524231-Macrophages,
pubmed-meshheading:11524231-Male,
pubmed-meshheading:11524231-Middle Aged,
pubmed-meshheading:11524231-Neoplasm Proteins,
pubmed-meshheading:11524231-RNA, Messenger,
pubmed-meshheading:11524231-Receptors, Interleukin-8B,
pubmed-meshheading:11524231-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11524231-Thyroid Diseases,
pubmed-meshheading:11524231-Thyroid Gland,
pubmed-meshheading:11524231-Thyroid Neoplasms,
pubmed-meshheading:11524231-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
GRO-alpha in normal and pathological thyroid tissues and its regulation in thyroid-derived cells.
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pubmed:affiliation |
Department of Surgery, Institute of Anatomy, University of Leipzig, Germany. ausg@medizin.uni-leipzig.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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