Source:http://linkedlifedata.com/resource/pubmed/id/11511685
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2001-8-20
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| pubmed:abstractText |
The objective of our study was to determine the tissue distribution and localization of ADAM-10 protein in human and bovine cartilage and the changes it undergoes with cartilage degeneration seen in osteoarthritis (OA) and under the influence of interleukin-1 (IL-1). Human normal and OA articular cartilage and bovine nasal cartilage cultured in the presence of IL-1alpha were processed for histology and immunohistochemistry. ADAM-10 protein was extracted from human cartilage and analyzed by Western blotting using anti-ADAM-10 antibodies. Fluor S Image analyzer and Quantity One software program were applied to quantify the total amount of ADAM-10. ADAM-10 protein was detected in both human and bovine cartilage. The strongest immunostaining was found in the cytoplasm and/or cell membranes of the superficial and upper middle layer of normal adult human cartilage, in the clusters and fibrillated areas of OA cartilage, and in IL-1alpha-stimulated bovine nasal cartilage. The distribution of ADAM-10 protein in bovine nasal cartilage was dependent on the length of exposure to IL-1alpha and corresponded to the areas of proteoglycan depletion. By Western blotting analysis of human cartilage, ADAM-10 was primarily detected in the membrane-enriched fraction and its levels were increased in degenerated and OA cartilage compared to normal cartilage. The results of this study suggest that ADAM-10 might be an important factor associated with cartilage degenerative processes. (J Histochem Cytochem 49:1165-1176, 2001)
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADAM 10 protein, Bos taurus,
http://linkedlifedata.com/resource/pubmed/chemical/ADAM Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ADAM10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid Precursor Protein Secretases,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0022-1554
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
49
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1165-76
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11511685-ADAM Proteins,
pubmed-meshheading:11511685-Adult,
pubmed-meshheading:11511685-Aged,
pubmed-meshheading:11511685-Aged, 80 and over,
pubmed-meshheading:11511685-Amyloid Precursor Protein Secretases,
pubmed-meshheading:11511685-Animals,
pubmed-meshheading:11511685-Antibodies, Monoclonal,
pubmed-meshheading:11511685-Antibody Specificity,
pubmed-meshheading:11511685-Blotting, Western,
pubmed-meshheading:11511685-Cartilage,
pubmed-meshheading:11511685-Cartilage, Articular,
pubmed-meshheading:11511685-Cattle,
pubmed-meshheading:11511685-Cell Membrane,
pubmed-meshheading:11511685-Female,
pubmed-meshheading:11511685-Humans,
pubmed-meshheading:11511685-Immunohistochemistry,
pubmed-meshheading:11511685-Interleukin-1,
pubmed-meshheading:11511685-Male,
pubmed-meshheading:11511685-Membrane Proteins,
pubmed-meshheading:11511685-Metalloendopeptidases,
pubmed-meshheading:11511685-Middle Aged,
pubmed-meshheading:11511685-Nose,
pubmed-meshheading:11511685-Osteoarthritis,
pubmed-meshheading:11511685-Up-Regulation
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| pubmed:year |
2001
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| pubmed:articleTitle |
ADAM-10 protein is present in human articular cartilage primarily in the membrane-bound form and is upregulated in osteoarthritis and in response to IL-1alpha in bovine nasal cartilage.
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| pubmed:affiliation |
Department of Biochemistry, Rush Medical College, Rush-Presbyterian-St Luke's Medical Center, 1653 W. Congress Parkway, Chicago, IL 60612, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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