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rdf:type |
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lifeskim:mentions |
umls-concept:C0004793,
umls-concept:C0006556,
umls-concept:C0017262,
umls-concept:C0079459,
umls-concept:C0185117,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0325089,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C2347040,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
2001-8-10
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA encoding feline granulocyte colony stimulating factor (fG-CSF) was cloned from alveolar macrophages using the reverse transcriptase-polymerase chain reaction. The cDNA is 949 bp in length and encodes a predicted mature protein of 174 amino acids. Recombinant fG-CSF was expressed as a glutathione S-transferase fusion and purified by affinity chromatography. Biological activity of the recombinant protein was demonstrated using the murine myeloblastic cell line GNFS-60, which showed an ED50 for fG-CSF of approximately 2 ng/ml.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
347-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11497496-Amino Acid Sequence,
pubmed-meshheading:11497496-Animals,
pubmed-meshheading:11497496-Base Sequence,
pubmed-meshheading:11497496-Cats,
pubmed-meshheading:11497496-Cattle,
pubmed-meshheading:11497496-Cell Line,
pubmed-meshheading:11497496-Chromatography,
pubmed-meshheading:11497496-Cloning, Molecular,
pubmed-meshheading:11497496-DNA, Complementary,
pubmed-meshheading:11497496-Glutathione Transferase,
pubmed-meshheading:11497496-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:11497496-Humans,
pubmed-meshheading:11497496-Macrophages, Alveolar,
pubmed-meshheading:11497496-Mice,
pubmed-meshheading:11497496-Molecular Sequence Data,
pubmed-meshheading:11497496-Rats,
pubmed-meshheading:11497496-Recombinant Fusion Proteins,
pubmed-meshheading:11497496-Recombinant Proteins,
pubmed-meshheading:11497496-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11497496-Sequence Homology, Amino Acid,
pubmed-meshheading:11497496-Sheep
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pubmed:year |
2001
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pubmed:articleTitle |
Isolation, nucleotide sequence and expression of a cDNA encoding feline granulocyte colony-stimulating factor.
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pubmed:affiliation |
Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK. s.dunham@vet.gla.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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