Linked Life Data

11494410

Source:http://linkedlifedata.com/resource/pubmed/id/11494410

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-8-8
pubmed:abstractText
Glial fibrillary acidic protein (GFAP) is the main component of intermediate filaments in astrocytes. To assess its function in astrocyte swelling, we compared astrocyte membrane properties and swelling in spinal cord slices of 8- to 10-day-old wild-type control (GFAP(+/+)) and GFAP-knockout (GFAP(-/-)) mice. Membrane currents and K(+) accumulation around astrocytes after a depolarizing pulse were studied using the whole-cell patch-clamp technique. In vivo cell swelling was studied in the cortex during spreading depression (SD) in 3 to 6-month-old animals. Swelling-induced changes of the extracellular space (ECS) diffusion parameters, i.e., volume fraction alpha and tortuosity lambda, were studied by the real-time iontophoretic tetramethylammonium (TMA(+)) method using TMA(+)-selective microelectrodes. Morphological analysis using confocal microscopy and quantification of xy intensity profiles in a confocal plane revealed a lower density of processes in GFAP(-/-) astrocytes than in GFAP(+/+) astrocytes. K(+) accumulation evoked by membrane depolarization was lower in the vicinity of GFAP(-/-) astrocytes than GFAP(+/+) astrocytes, suggesting the presence of a larger ECS around GFAP(-/-) astrocytes. Astrocyte swelling evoked by application of 50 mM K(+) or by hypotonic solution (HS) produced a larger increase in [K(+)](e) around GFAP(+/+) astrocytes than around GFAP(-/-) astrocytes. No differences in alpha and lambda in the spinal cord or cortex of GFAP(+/+) and GFAP(-/-) mice were found; however, the application of either 50 mM K(+) or HS in spinal cord, or SD in cortex, evoked a large decrease in alpha and an increase in lambda in GFAP(+/+) mice only. Slower swelling in GFAP(-/-) astrocytes indicates that GFAP and intermediate filaments play an important role in cell swelling during pathological states.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0894-1491
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-203
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11494410-Animals, pubmed-meshheading:11494410-Astrocytes, pubmed-meshheading:11494410-Cell Membrane, pubmed-meshheading:11494410-Cell Membrane Permeability, pubmed-meshheading:11494410-Cell Size, pubmed-meshheading:11494410-Cortical Spreading Depression, pubmed-meshheading:11494410-Diffusion, pubmed-meshheading:11494410-Extracellular Space, pubmed-meshheading:11494410-Fluorescent Dyes, pubmed-meshheading:11494410-Glial Fibrillary Acidic Protein, pubmed-meshheading:11494410-Intermediate Filaments, pubmed-meshheading:11494410-Isoquinolines, pubmed-meshheading:11494410-Membrane Potentials, pubmed-meshheading:11494410-Mice, pubmed-meshheading:11494410-Mice, Knockout, pubmed-meshheading:11494410-Osmotic Pressure, pubmed-meshheading:11494410-Patch-Clamp Techniques, pubmed-meshheading:11494410-Potassium, pubmed-meshheading:11494410-Somatosensory Cortex, pubmed-meshheading:11494410-Spinal Cord
pubmed:year
2001
pubmed:articleTitle
Effect of elevated K(+), hypotonic stress, and cortical spreading depression on astrocyte swelling in GFAP-deficient mice.
pubmed:affiliation
Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't