Source:http://linkedlifedata.com/resource/pubmed/id/11485630
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
2001-8-3
|
| pubmed:abstractText |
A phase I clinical trial was conducted in which recombinant adenovirus containing the cystic fibrosis trans-membrane regulator (CFTR) (Ad2/CFTR) was administered by bronchoscopic instillation or aerosolization to the lungs of cystic fibrosis (CF) patients. In this paper, we evaluate the efficiency of Ad2/CFTR-mediated transduction of bronchial airway cells. The ability of an Ad2/CFTR vector to transduce airway cells was first evaluated in patients to whom the vector was administered by bronchoscopic instillation. Cells at the administration site were collected 2 days after treatment by bronchoscopic brushing. Ad2-specific CFTR DNA was detected in four of five individuals by PCR, and Ad2-specific CFTR RNA was detected in three of five individuals by RT-PCR. Ad2/CFTR-mediated transduction of airway epithelial cells was then determined in CF individuals receiving this vector by aerosol inhalation. Ad2-specific CFTR DNA was detected in 13 of 13 individuals 2 days after aerosolization, and in 3 of 5 individuals 7 days after aerosolization. Ad2-specific RNA was detected in 4 of 13 individuals on day 2, but was not detected in the 5 individuals tested on day 7. The percentage of airway epithelial cells containing nuclear-localized vector DNA was < or =2.4% as determined by fluorescence in situ hybridization (FISH). However, in some cases, a high percentage of nonepithelial mononuclear cells or squamous metaplastic epithelial cells was infected with the adenoviral vector. In conclusion, aerosol administration is a feasible means to distribute adenoviral vectors throughout the conducting airways, but improvements in adenovirus-mediated transduction of airway epithelial cells are necessary before gene therapy for CF will be effective.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CFTR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cystic Fibrosis Transmembrane...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1043-0342
|
| pubmed:author |
pubmed-author:BalfourRR,
pubmed-author:DorkinHH,
pubmed-author:JosephP MPM,
pubmed-author:LapeyAA,
pubmed-author:MeekerD PDP,
pubmed-author:MorrisJ EJE,
pubmed-author:O'SullivanB PBP,
pubmed-author:PavelkaKK,
pubmed-author:PerriconeM AMA,
pubmed-author:PlogM SMS,
pubmed-author:SmithA EAE,
pubmed-author:St GeorgeJ AJA,
pubmed-author:WadsworthS CSC
|
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1383-94
|
| pubmed:dateRevised |
2006-4-21
|
| pubmed:meshHeading |
pubmed-meshheading:11485630-Adenoviridae,
pubmed-meshheading:11485630-Administration, Inhalation,
pubmed-meshheading:11485630-Adolescent,
pubmed-meshheading:11485630-Adult,
pubmed-meshheading:11485630-Bronchoscopy,
pubmed-meshheading:11485630-Cystic Fibrosis,
pubmed-meshheading:11485630-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:11485630-DNA, Recombinant,
pubmed-meshheading:11485630-Female,
pubmed-meshheading:11485630-Genetic Vectors,
pubmed-meshheading:11485630-Humans,
pubmed-meshheading:11485630-In Situ Hybridization, Fluorescence,
pubmed-meshheading:11485630-Instillation, Drug,
pubmed-meshheading:11485630-Male,
pubmed-meshheading:11485630-Polymerase Chain Reaction,
pubmed-meshheading:11485630-RNA, Messenger,
pubmed-meshheading:11485630-Recombinant Proteins,
pubmed-meshheading:11485630-Respiratory Mucosa,
pubmed-meshheading:11485630-Time Factors,
pubmed-meshheading:11485630-Transduction, Genetic,
pubmed-meshheading:11485630-Transfection
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Aerosol and lobar administration of a recombinant adenovirus to individuals with cystic fibrosis. II. Transfection efficiency in airway epithelium.
|
| pubmed:affiliation |
Genzyme Corporation, Framingham, MA 01701, USA. michael. perricone@genyme.com
|
| pubmed:publicationType |
Journal Article,
Clinical Trial,
Multicenter Study,
Clinical Trial, Phase I
|