Linked Life Data

114522

Source:http://linkedlifedata.com/resource/pubmed/id/114522

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1979-12-18
pubmed:databankReference
pubmed:abstractText
The DNA sequence of a cloned gene specifying Drosophila tRNA2Arg has been determined. Transcription of this gene in extracts from Xenopus germinal vesicles yielded a precursor RNA containing a seven-nucleotide leader sequence at the 5' side of the mature tRNA with pppGp as the 5'-terminal nucleotide. Transcription continues for at least eight nucleotides past the 3' terminus of the coding region of the gene and terminates over a stretch of seven T residues. The isolation of the primary transcript was facilitated by incorporation of 5'-(gamma-S)GTP into the 5' terminus of the precursor RNA and by its retention on mercury-agarose. The Drosophila tRNAArg gene does not contain an intervening sequence nor the C-C-A sequence corresponding to the 3' terminus of the mature tRNA. The nucleotide sequence of Drosophila tRNAArg formed in the in vitro system differs only in four positions from that of mouse tRNAArg.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
254
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10290-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.