Linked Life Data

11449408

Source:http://linkedlifedata.com/resource/pubmed/id/11449408

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-7-12
pubmed:abstractText
Flow cytometry has become a powerful technique to measure intracellular cytokine production in lymphocytes and monocytes. Appropriate inhibition of the secretion of the produced cytokines is required for studying intracellular cytokine expression. The aim of this study was to compare the capacity of cytokine secretion inhibitors, monensin and brefeldin A, in order to trap cytokine production (interleukin-1 beta [IL-1beta], IL-6, tumor necrosis factor-alpha [TNF-alpha]) within peripheral blood monocytes. A two-color flow cytometric technique was used to measure intracellular spontaneous and lipopolysaccharide (LPS)-stimulated IL-1beta, IL-6, and TNF-alpha production in monocytes (CD14+) of whole blood cultures. The viability of monensin-treated monocytes was slightly lower than that of brefeldin A-inhibited monocytes, as measured with propidium iodide (PI). The percentage of IL-6 and TNF-alpha-producing monocytes after 8 h of culture without stimulation revealed significant lower values for monensin-treated than for brefeldin A-treated monocytes. The percentages for stimulated cells did not differ. The spontaneous intracellular production in molecules of equivalent soluble fluorochrome units (MESF) of IL-1beta, IL-6, and TNF-alpha after 8 h of culture was higher in brefeldin A than in monensin-inhibited monocytes. The LPS-stimulated intracellular production of IL-1beta, IL-6, and TNF-alpha was increased in brefeldin A-inhibited monocytes. In conclusion, for flow cytometric determination of intracellular monocytic cytokines (IL-1beta, IL-6, and TNF-alpha), brefeldin A is a more potent, effective, and less toxic inhibitor of cytokine secretion than monensin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0196-4763
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
172-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11449408-Adult, pubmed-meshheading:11449408-Antigens, CD14, pubmed-meshheading:11449408-Arthritis, Rheumatoid, pubmed-meshheading:11449408-Brefeldin A, pubmed-meshheading:11449408-Cell Survival, pubmed-meshheading:11449408-Cells, Cultured, pubmed-meshheading:11449408-Female, pubmed-meshheading:11449408-Flow Cytometry, pubmed-meshheading:11449408-Humans, pubmed-meshheading:11449408-Interleukin-1, pubmed-meshheading:11449408-Interleukin-6, pubmed-meshheading:11449408-Ionophores, pubmed-meshheading:11449408-Lipopolysaccharides, pubmed-meshheading:11449408-Male, pubmed-meshheading:11449408-Middle Aged, pubmed-meshheading:11449408-Monensin, pubmed-meshheading:11449408-Monocytes, pubmed-meshheading:11449408-Protein Synthesis Inhibitors, pubmed-meshheading:11449408-Time Factors, pubmed-meshheading:11449408-Tumor Necrosis Factor-alpha
pubmed:year
2001
pubmed:articleTitle
Evaluation of monensin and brefeldin A for flow cytometric determination of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha in monocytes.
pubmed:affiliation
Department of Immunology, Allergology and Rheumatology, University of Antwerp, Antwerp, Belgium.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't