Linked Life Data

11389003

Source:http://linkedlifedata.com/resource/pubmed/id/11389003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-6-4
pubmed:abstractText
The earliest stages of lymphoid commitment from human pluripotent hematopoietic stem cells have not been defined. A clonogenic subpopulation of CD34(+)CD38(-) cord blood cells were identified that expressed high levels of the CD7 antigen and possessed only lymphoid potential. CD34(+)CD38(-)CD7(+) (CD7(+)) cells uniformly coexpressed CD45RA and HLA-DR; c-kit and Thy-1 expression was absent to low. Clonal analysis demonstrated that single CD7(+) cells could generate B cells, natural killer cells, and dendritic cells but were devoid of myeloid or erythroid potential. In contrast, control CD34(+)CD38(-)CD7(-) (CD7(-)) cells generated both lymphoid and myelo-erythroid cells. The lymphoid potential (generation of lymphoid progeny in bulk and single cell cultures) of CD7(+) cells was equivalent to that of the pluripotent CD7(-) cells. RNA expression studies showed that CD7(+) cells expressed PU.1 and GATA-3, but did not express Pax-5, terminal deoxynucleotide transferase, or CD3epsilon. In contrast to the previously described murine common lymphoid progenitor, the alpha chain of the receptor for interleukin-7 was not detected by fluorescence-activated cell sorting analysis or RNA polymerase chain reaction in CD7(+) cells. These studies identify a clonogenic lymphoid progenitor with both B-cell and natural killer cell lineage potential with a molecular profile that suggests a developmental stage more primitive than previously identified lymphoid progenitors. The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells, thus allowing the study of regulation of early human lymphopoiesis and providing an alternative to pluripotent stem cells for genetic manipulation and transplantation. (Blood. 2001;97:3683-3690)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3683-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11389003-ADP-ribosyl Cyclase, pubmed-meshheading:11389003-Antigens, CD, pubmed-meshheading:11389003-Antigens, CD34, pubmed-meshheading:11389003-Antigens, CD38, pubmed-meshheading:11389003-Antigens, CD7, pubmed-meshheading:11389003-Antigens, Differentiation, pubmed-meshheading:11389003-B-Lymphocytes, pubmed-meshheading:11389003-Cell Differentiation, pubmed-meshheading:11389003-Cell Lineage, pubmed-meshheading:11389003-Clone Cells, pubmed-meshheading:11389003-Dendritic Cells, pubmed-meshheading:11389003-Fetal Blood, pubmed-meshheading:11389003-Hematopoiesis, pubmed-meshheading:11389003-Hematopoietic Stem Cells, pubmed-meshheading:11389003-Humans, pubmed-meshheading:11389003-Immunophenotyping, pubmed-meshheading:11389003-Killer Cells, Natural, pubmed-meshheading:11389003-Lymphocyte Subsets, pubmed-meshheading:11389003-Lymphocytes, pubmed-meshheading:11389003-Membrane Glycoproteins, pubmed-meshheading:11389003-NAD+ Nucleosidase, pubmed-meshheading:11389003-RNA, Messenger
pubmed:year
2001
pubmed:articleTitle
Identification of a novel, human multilymphoid progenitor in cord blood.
pubmed:affiliation
Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital of Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't