Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-5-22
pubmed:abstractText
This report describes stroma-based and stroma-free cultures that maintain long-term engrafting hematopoietic cells for at least 14 days ex vivo. Umbilical cord blood (UCB) CD34(+) cells were cultured in transwells above AFT024 feeders with fetal-liver-tyrosine-kinase (FL) + stem cell factor (SCF) + interleukin 7 (IL-7), or FL + thrombopoietin (Tpo). CD34(+) progeny were transplanted into nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice or preimmune fetal sheep. SCID repopulating cells (SRC) with multilineage differentiation potential were maintained in FL-SCF-IL-7 or FL-Tpo containing cultures for up to 28 days. Marrow from mice highly engrafted with uncultured or expanded cells induced multilineage human hematopoiesis in 50% of secondary but not tertiary recipients. Day 7 expanded cells engrafted primary, secondary, and tertiary fetal sheep. Day 14 expanded cells, although engrafting primary and to a lesser degree secondary fetal sheep, failed to engraft tertiary recipients. SRC that can be transferred to secondary recipients were maintained for at least 14 days in medium containing glycosaminoglycans and cytokines found in stromal supernatants. This is the first demonstration that ex vivo culture in stroma-noncontact and stroma-free cultures maintains "long-term" engrafting cells, defined by their capacity to engraft secondary or tertiary hosts. (Blood. 2001;97:3441-3449)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3441-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11369635-Animals, pubmed-meshheading:11369635-Cell Culture Techniques, pubmed-meshheading:11369635-Cell Differentiation, pubmed-meshheading:11369635-Coculture Techniques, pubmed-meshheading:11369635-Diabetes Mellitus, Type 1, pubmed-meshheading:11369635-Fetal Blood, pubmed-meshheading:11369635-Hematopoietic Stem Cell Transplantation, pubmed-meshheading:11369635-Humans, pubmed-meshheading:11369635-Interleukin-7, pubmed-meshheading:11369635-Liver, pubmed-meshheading:11369635-Mice, pubmed-meshheading:11369635-Mice, Inbred NOD, pubmed-meshheading:11369635-Mice, SCID, pubmed-meshheading:11369635-Protein-Tyrosine Kinases, pubmed-meshheading:11369635-Sheep, pubmed-meshheading:11369635-Stem Cell Factor, pubmed-meshheading:11369635-Stromal Cells, pubmed-meshheading:11369635-Thrombopoietin, pubmed-meshheading:11369635-Transplantation, Heterologous
pubmed:year
2001
pubmed:articleTitle
Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system.
pubmed:affiliation
Stem Cell Institute, the Department of Medicine and Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't