Linked Life Data

11335962

Source:http://linkedlifedata.com/resource/pubmed/id/11335962

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-5-3
pubmed:abstractText
Loss of heterozygosity (LOH) at the 3p region is found in up to 50% of epithelial ovarian neoplasms. The von Hippel-Lindau (VHL) gene at the 3p25 locus is one of the tumor-suppressor genes located at 3p. The role, if any, of the VHL gene locus is not clear in ovarian carcinogenesis. We analyzed primary and metastatic ovarian clear-cell carcinomas (OCCC) for LOH at 3p25 to determine its frequency and its diagnostic utility as an adjunctive tool in the differential diagnosis of metastatic clear-cell carcinomas. Microdissection followed by single-step DNA extraction and polymerase chain reaction (PCR) amplification, using two polymorphic markers flanking the VHL gene locus, was done on archival histology and cytology samples from 9 patients with metastatic OCCC. Of the informative cases, 43% of the metastatic and 50% of the primary OCCC showed LOH. LOH at the VHL gene locus is not uncommon in clear-cell ovarian carcinoma. LOH at 3p25 in cytologic specimens may be a valuable adjunct in the diagnosis of OCCC metastasis in cytologically equivocal cases. OCCC should enter the differential in clear-cell carcinomas of unknown primary that show LOH at 3p25. Published 2001 Wiley-Liss, Inc.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
8755-1039
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
328-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11335962-Adenocarcinoma, Clear Cell, pubmed-meshheading:11335962-Chromosomes, Human, Pair 3, pubmed-meshheading:11335962-Diagnosis, Differential, pubmed-meshheading:11335962-Dissection, pubmed-meshheading:11335962-Female, pubmed-meshheading:11335962-Genes, Tumor Suppressor, pubmed-meshheading:11335962-Humans, pubmed-meshheading:11335962-Ligases, pubmed-meshheading:11335962-Loss of Heterozygosity, pubmed-meshheading:11335962-Ovarian Neoplasms, pubmed-meshheading:11335962-Polymerase Chain Reaction, pubmed-meshheading:11335962-Proteins, pubmed-meshheading:11335962-Retrospective Studies, pubmed-meshheading:11335962-Tumor Markers, Biological, pubmed-meshheading:11335962-Tumor Suppressor Proteins, pubmed-meshheading:11335962-Ubiquitin-Protein Ligases, pubmed-meshheading:11335962-Von Hippel-Lindau Tumor Suppressor Protein
pubmed:year
2001
pubmed:articleTitle
Detection of loss of heterozygosity at chromosome 3p25-26 in primary and metastatic ovarian clear-cell carcinoma: utilization of microdissection and polymerase chain reaction in archival tissues.
pubmed:affiliation
Cytopathology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article