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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-4-25
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pubmed:abstractText |
Using bioinformatics approaches, 34 potential multidrug resistance (MDR) transporter sequences representing 4 different transporter families were identified in the unannotated Enterococcus faecalis database (TIGR). A functional genomics campaign generating single-gene insertional disruptions revealed several genes whose absence confers significant hypersensitivities to known antimicrobials. We constructed specific strains, disrupted in a variety of previously unpublished, putative MDR transporter genes, as tools to improve the success of whole-cell antimicrobial screening and discovery. Each of the potential transporters was inactivated at the gene level and then phenotypically characterized, both with single disruption mutants and with 2-gene mutants built upon a delta norA deleted strain background.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1464-1801
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179-84
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11321571-ATP-Binding Cassette Transporters,
pubmed-meshheading:11321571-Anti-Bacterial Agents,
pubmed-meshheading:11321571-Bacterial Proteins,
pubmed-meshheading:11321571-Carrier Proteins,
pubmed-meshheading:11321571-Databases as Topic,
pubmed-meshheading:11321571-Drug Design,
pubmed-meshheading:11321571-Drug Resistance, Microbial,
pubmed-meshheading:11321571-Enterococcus faecalis,
pubmed-meshheading:11321571-Gene Deletion,
pubmed-meshheading:11321571-Genomics,
pubmed-meshheading:11321571-Multidrug Resistance-Associated Proteins,
pubmed-meshheading:11321571-Mutagenesis,
pubmed-meshheading:11321571-Structure-Activity Relationship
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pubmed:year |
2001
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pubmed:articleTitle |
Enterococcus faecalis multi-drug resistance transporters: application for antibiotic discovery.
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pubmed:affiliation |
Phytera, Inc, Worcester, MA 01605, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Review
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