Linked Life Data

11290588

Source:http://linkedlifedata.com/resource/pubmed/id/11290588

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-4-6
pubmed:abstractText
Cytokine-mobilized peripheral blood hematopoietic stem cells (MPB HSC) are widely used for transplantation in the treatment of malignancies, but the mechanism of HSC mobilization is unclear. Although many HSC in bone marrow (BM) cycle rapidly and expand their numbers in response to cytoreductive agents, such as cyclophosphamide (CY), and cytokines, such as granulocyte colony-stimulating factor (G-CSF), MPB HSC are almost all in the G(0) or G(1) phase of the cell cycle. This has raised the question of whether a subset of noncycling BM HSC is selectively released, or whether cycling BM HSC are mobilized after M phase, but before the next S phase of the cell cycle. To distinguish between these possibilities, mice were treated with one dose of CY followed by daily doses of G-CSF, and dividing cells were marked by administration of bromodeoxyuridine (BrdU) during the interval that BM HSC are expanding. After CY and 4 days of G-CSF, 98.5% of the 2n DNA content long-term repopulating MPB (LT)-HSC stained positively for BrdU, and therefore derived from cells that divided during the treatment interval. Next, LT-HSC from mice previously treated with a single dose of CY, which kills cycling cells, and 3 daily doses of G-CSF, were nearly all killed by a second dose of CY, suggesting that CY/G-CSF causes virtually all LT-HSC to cycle. Analysis of cyclin D2 messenger RNA (mRNA) expression and total RNA content of MPB HSC suggests that these cells are mostly in G(1) phase. After CY/G-CSF treatment, virtually all BM LT-HSC enter the cell cycle; some of these HSC then migrate into the blood, specifically after M phase, and are rapidly recruited to particular hematopoietic organs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2278-85
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11290588-Animals, pubmed-meshheading:11290588-Blood Cells, pubmed-meshheading:11290588-Bone Marrow, pubmed-meshheading:11290588-Cell Lineage, pubmed-meshheading:11290588-Cell Movement, pubmed-meshheading:11290588-Cells, Cultured, pubmed-meshheading:11290588-Cyclin D2, pubmed-meshheading:11290588-Cyclins, pubmed-meshheading:11290588-Cyclophosphamide, pubmed-meshheading:11290588-DNA Replication, pubmed-meshheading:11290588-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:11290588-Hematopoietic Stem Cell Mobilization, pubmed-meshheading:11290588-Hematopoietic Stem Cells, pubmed-meshheading:11290588-Metaphase, pubmed-meshheading:11290588-Mice, pubmed-meshheading:11290588-Mice, Inbred C3H, pubmed-meshheading:11290588-Mice, Inbred C57BL, pubmed-meshheading:11290588-Mice, Transgenic, pubmed-meshheading:11290588-Organ Specificity, pubmed-meshheading:11290588-RNA, Messenger, pubmed-meshheading:11290588-Recombinant Fusion Proteins, pubmed-meshheading:11290588-Spleen
pubmed:year
2001
pubmed:articleTitle
Cyclophosphamide/granulocyte colony-stimulating factor causes selective mobilization of bone marrow hematopoietic stem cells into the blood after M phase of the cell cycle.
pubmed:affiliation
Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't