Source:http://linkedlifedata.com/resource/pubmed/id/11287212
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2001-4-5
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| pubmed:abstractText |
We previously cloned cDNAs for all the members (A, B and C) of Xenopus aldolase gene family, and using in vitro transcribed RNAs as references, performed quantitative studies of the expression of three aldolase mRNAs in embryos and adult tissues. A Xenopus egg contains ca. 60 pg aldolase A mRNA and ca. 45 pg aldolase C mRNA, but contains only ca. 1.5 pg aldolase B mRNA. The percent composition of three aldolase mRNAs (A:B:C) changes from 56:1.5:42.5 (fertilized egg) to 54:10:36 (gastrula), to 71:14.5:14.5 (neurula) and to 73:20:7 (tadpole) during development. These results are compatible with the previous results of zymogram analysis that aldolases A and C are the major aldolases in early embryos, whose development proceeds depending on yolk as the only energy source. Aldolase B mRNA is expressed only late in development in tissues such as pronephros, liver rudiment and proctodeum which are necessary for the future dietary fructose metabolism, and the expression pattern is consistent to that in adult tissues. We also show that three aldolase genes are localized on different chromosomes as single copy genes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0925-4773
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
102
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
283-7
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11287212-Animals,
pubmed-meshheading:11287212-Blotting, Northern,
pubmed-meshheading:11287212-Chromosomes,
pubmed-meshheading:11287212-DNA, Complementary,
pubmed-meshheading:11287212-Fructose-Bisphosphate Aldolase,
pubmed-meshheading:11287212-In Situ Hybridization, Fluorescence,
pubmed-meshheading:11287212-Karyotyping,
pubmed-meshheading:11287212-Microscopy, Fluorescence,
pubmed-meshheading:11287212-Models, Biological,
pubmed-meshheading:11287212-RNA, Messenger,
pubmed-meshheading:11287212-Time Factors,
pubmed-meshheading:11287212-Tissue Distribution,
pubmed-meshheading:11287212-Transcription, Genetic,
pubmed-meshheading:11287212-Xenopus laevis
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| pubmed:year |
2001
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| pubmed:articleTitle |
Quantitative expression studies of aldolase A, B and C genes in developing embryos and adult tissues of Xenopus laevis.
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| pubmed:affiliation |
Laboratory of Molecular Embryology, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo ku, 113-0033, Tokyo, Japan.
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| pubmed:publicationType |
Journal Article
|