Linked Life Data

11278892

Source:http://linkedlifedata.com/resource/pubmed/id/11278892

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2001-5-23
pubmed:databankReference
pubmed:abstractText
Heparan sulfate, one of the most abundant components of the cell surface and the extracellular matrix, is involved in a variety of biological processes such as growth factor signaling, cell adhesion, and enzymatic catalysis. The heparan sulfate chains have markedly heterogeneous structures in which distinct sequences of sulfate groups determine specific binding properties. Sulfation at each different position of heparan sulfate is catalyzed by distinct enzymes, sulfotransferases. In this study, we identified and characterized Drosophila heparan sulfate 6-O-sulfotransferase (dHS6ST). The deduced primary structure of dHS6ST exhibited several common features found in those of mammalian HS6STs. We confirmed that, when the protein encoded by the cDNA was expressed in COS-7 cells, it showed HS6ST activity. Whole mount in situ hybridization revealed highly specific expression of dHS6ST mRNA in embryonic tracheal cells. The spatial and temporal pattern of dHS6ST expression in these cells clearly resembles that of the Drosophila fibroblast growth factor (FGF) receptor, breathless (btl). RNA interference experiments demonstrated that reduced dHS6ST activity caused embryonic lethality and disruption of the primary branching of the tracheal system. These phenotypes were reminiscent of the defects observed in mutants of FGF signaling components. We also show that FGF-dependent mitogen-activated protein kinase activation is significantly reduced in dHS6ST double-stranded RNA-injected embryos. These findings indicate that dHS6ST is required for tracheal development in Drosophila and suggest the evolutionally conserved roles of 6-O-sulfated heparan sulfate in FGF signaling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17014-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11278892-Amino Acid Sequence, pubmed-meshheading:11278892-Animals, pubmed-meshheading:11278892-Base Sequence, pubmed-meshheading:11278892-COS Cells, pubmed-meshheading:11278892-Cercopithecus aethiops, pubmed-meshheading:11278892-Conserved Sequence, pubmed-meshheading:11278892-Drosophila melanogaster, pubmed-meshheading:11278892-Embryo, Nonmammalian, pubmed-meshheading:11278892-Exons, pubmed-meshheading:11278892-Gene Expression Regulation, Developmental, pubmed-meshheading:11278892-Gene Expression Regulation, Enzymologic, pubmed-meshheading:11278892-Genes, Insect, pubmed-meshheading:11278892-Humans, pubmed-meshheading:11278892-Mammals, pubmed-meshheading:11278892-Mice, pubmed-meshheading:11278892-Molecular Sequence Data, pubmed-meshheading:11278892-RNA, Messenger, pubmed-meshheading:11278892-Recombinant Proteins, pubmed-meshheading:11278892-Sequence Alignment, pubmed-meshheading:11278892-Sequence Homology, Amino Acid, pubmed-meshheading:11278892-Sulfotransferases, pubmed-meshheading:11278892-Trachea, pubmed-meshheading:11278892-Transcription, Genetic, pubmed-meshheading:11278892-Transfection
pubmed:year
2001
pubmed:articleTitle
Drosophila heparan sulfate 6-O-sulfotransferase (dHS6ST) gene. Structure, expression, and function in the formation of the tracheal system.
pubmed:affiliation
Department of Biology, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't