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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-3-14
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pubmed:abstractText |
In a previous study, it was found that exposure to tumor necrosis factor-alpha (TNF-alpha) potentiated the electrophysiological response to carbachol in a time-dependent and cycloheximide-sensitive manner. It was deduced that the potentiation could be due to protein kinase C activity because of increased 1,2-diacylglycerol. It was also observed that propranolol could decrease the electrophysiological response to carbachol (Oprins JC, Meijer HP, and Groot JA. Am J Physiol Cell Physiol 278: C463-C472, 2000). The aim of the present study was to investigate whether the phospholipase D (PLD) pathway plays a role in the carbachol response and the potentiating effect of TNF-alpha. The transphosphatidylation reaction in the presence of the primary alcohol 1-butanol [leading to stable phosphatidylbutanol (Pbut) formation] was used to measure activity of PLD. The phosphatidic acid (PA) levels were also measured. Muscarinic stimulation resulted in an increased formation of Pbut and PA. TNF-alpha decreased levels of PA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Butanol,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Cholinergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Diacylglycerol Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Propranolol,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidinones,
http://linkedlifedata.com/resource/pubmed/chemical/R 59022,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide I
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0363-6143
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C789-95
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11245595-1-Butanol,
pubmed-meshheading:11245595-Adrenergic beta-Antagonists,
pubmed-meshheading:11245595-Carbachol,
pubmed-meshheading:11245595-Carcinogens,
pubmed-meshheading:11245595-Chlorides,
pubmed-meshheading:11245595-Cholinergic Agonists,
pubmed-meshheading:11245595-Chromatography, Thin Layer,
pubmed-meshheading:11245595-Diacylglycerol Kinase,
pubmed-meshheading:11245595-Diglycerides,
pubmed-meshheading:11245595-Dose-Response Relationship, Drug,
pubmed-meshheading:11245595-Enzyme Inhibitors,
pubmed-meshheading:11245595-HT29 Cells,
pubmed-meshheading:11245595-Humans,
pubmed-meshheading:11245595-Indoles,
pubmed-meshheading:11245595-Intestinal Mucosa,
pubmed-meshheading:11245595-Maleimides,
pubmed-meshheading:11245595-Phorbol Esters,
pubmed-meshheading:11245595-Phospholipase D,
pubmed-meshheading:11245595-Propranolol,
pubmed-meshheading:11245595-Protein Kinase C,
pubmed-meshheading:11245595-Pyrimidinones,
pubmed-meshheading:11245595-Receptors, Muscarinic,
pubmed-meshheading:11245595-Signal Transduction,
pubmed-meshheading:11245595-Thiazoles,
pubmed-meshheading:11245595-Tumor Necrosis Factor-alpha
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pubmed:year |
2001
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pubmed:articleTitle |
PLD pathway involved in carbachol-induced Cl- secretion: possible role of TNF-alpha.
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pubmed:affiliation |
Swammerdam Institute for Life Sciences, University of Amsterdam, P.O. Box 94084, 1090 GB Amsterdam, The Netherlands. oprins@bio.uva.nl
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pubmed:publicationType |
Journal Article
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