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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15-16
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
Escherichia coli O157:H7 produces two forms of verotoxin (VT), VT1 and VT2, which cause hemorrhagic colitis with development, in some cases, of hemolytic uremic syndrome. These toxins consist of an enzymatically active A subunit and pentamers of B subunit responsible for their binding to host cells. We used the secretion-expression system of Bacillus brevis to produce recombinant VT1B and VT2B. The secreted B subunits were purified and sequenced to verify their structure. Receptor-binding showed that rVT1B but not rVT2B bound to Gb3-receptor. When mice were nasally immunized with rVT1B or rVT2B together with a nontoxic mutant of cholera toxin (mCT) or native cholera toxin (nCT) as adjuvants, serum IgG and mucosal IgA antibody responses to VT1B were induced. The VT1B-specific antibodies prevented VT1B binding to its Gb3 receptor. In contrast, poor serum and no mucosal VT2B-specific antibodies but brisk CTB-specific antibody responses were induced by nasal immunization with rVT2B in the presence of mCT or nCT. These results show that nasal immunization with rVTB and mCT as a nontoxic mucosal adjuvant is an effective regimen for the induction of VT1B but not VT2B antibody responses which inhibit VT1B binding to Gb3 receptor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Shiga Toxin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Shiga Toxin 2,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2061-70
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11228378-Adjuvants, Immunologic,
pubmed-meshheading:11228378-Administration, Intranasal,
pubmed-meshheading:11228378-Amino Acid Sequence,
pubmed-meshheading:11228378-Animals,
pubmed-meshheading:11228378-Antibodies, Bacterial,
pubmed-meshheading:11228378-Bacillus,
pubmed-meshheading:11228378-Bacterial Vaccines,
pubmed-meshheading:11228378-Base Sequence,
pubmed-meshheading:11228378-Cholera Toxin,
pubmed-meshheading:11228378-DNA Primers,
pubmed-meshheading:11228378-Escherichia coli O157,
pubmed-meshheading:11228378-Genetic Vectors,
pubmed-meshheading:11228378-Mice,
pubmed-meshheading:11228378-Mice, Inbred BALB C,
pubmed-meshheading:11228378-Molecular Sequence Data,
pubmed-meshheading:11228378-Neutralization Tests,
pubmed-meshheading:11228378-Plasmids,
pubmed-meshheading:11228378-Protein Subunits,
pubmed-meshheading:11228378-Shiga Toxin 1,
pubmed-meshheading:11228378-Shiga Toxin 2,
pubmed-meshheading:11228378-Vaccines, Synthetic
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pubmed:year |
2001
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pubmed:articleTitle |
Nasal immunization with E. coli verotoxin 1 (VT1)-B subunit and a nontoxic mutant of cholera toxin elicits serum neutralizing antibodies.
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pubmed:affiliation |
JCR Biopharmaceuticals Incorporated, , San Diego, CA 92121-1194, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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