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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-2-12
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pubmed:abstractText |
We compared the effects of LPSs from P. gingivalis and P. heparinolytica on the induction of tumor necrosis factor-alpha (TNF-alpha) production by murine peritoneal macrophages. The lipopolysaccharide (LPS) preparation from P. gingivalis showed a typical ladder pattern in SDS-PAGE, whereas that from P. heparinolytica formed several stained bands without a ladder pattern. When the macrophages from C3H/HeJ mice were incubated with P. gingivalis LPS, the level of TNF-alpha released in the culture supernatants was significantly higher than that with P. heparinolytica. All tested reagents except genistein strongly inhibited the production of TNF-alpha by the macrophages after induction by either LPS. These results suggested the following possibilities, i) the induction level of TNF-alpha by P. heparinolytica is similar to that of Salmonella minnesota, ii) tyrosine phosphorylation is not the only pathway for the TNF-alpha production induced by these LPSs, iii) different regulatory mechanisms are involved in TNF-alpha production in P. gingivalis LPS stimulated and P. heparinolytica LPS stimulated cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0040-8891
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
135-40
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11212586-Animals,
pubmed-meshheading:11212586-Deoxycholic Acid,
pubmed-meshheading:11212586-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11212586-Enzyme Inhibitors,
pubmed-meshheading:11212586-Genistein,
pubmed-meshheading:11212586-Lipopolysaccharides,
pubmed-meshheading:11212586-Macrophages, Peritoneal,
pubmed-meshheading:11212586-Mice,
pubmed-meshheading:11212586-Mice, Inbred C3H,
pubmed-meshheading:11212586-Mice, Inbred Strains,
pubmed-meshheading:11212586-Phosphorylation,
pubmed-meshheading:11212586-Porphyromonas gingivalis,
pubmed-meshheading:11212586-Prevotella,
pubmed-meshheading:11212586-Protein-Tyrosine Kinases,
pubmed-meshheading:11212586-Salmonella,
pubmed-meshheading:11212586-Sodium Dodecyl Sulfate,
pubmed-meshheading:11212586-Tumor Necrosis Factor-alpha,
pubmed-meshheading:11212586-Tyrosine
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pubmed:year |
2000
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pubmed:articleTitle |
Differences in TNF-alpha producing activity from murine peritoneal macrophages induced by lipopolysaccharides of Prevotella heparinolytica and Porphyromonas gingivalis.
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pubmed:affiliation |
Department of Oral Microbiology, Matsumoto Dental University, 1780, Hirooka-Gobara, Shiojiri-shi, Nagano 399-0781, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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