Source:http://linkedlifedata.com/resource/pubmed/id/11139078
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2001-1-2
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| pubmed:abstractText |
Proliferating cell nuclear antigen (PCNA) is an essential component of the DNA replication and repair machinery in the domain Eucarya. Eukaryotes and euryarchaeotes, which belong to one subdomain of Archaea, possess a single PCNA homologue, whereas two distinct PCNA homologues have been identified from Sulfolobus solfataricus, which belongs to the other archaeal subdomain, Crenarchaeota. We have cloned and sequenced two genes of PCNA homologues from the thermoacidophilic crenarchaeon Sulfurisphaera ohwakuensis. These genes, referred to as the Soh PCNA A gene and the Soh PCNA B gene, were found to encode 245 amino acids (aa) (27kDa) and 248 aa (27kDa), respectively. In deduced amino acid sequences of both PCNA homologues, the motif L/I-A-P-K/R, implicated in binding of PCNA with replication factor C (RFC), was identified. Phylogenetic analysis of all available archaeal PCNA homologues suggests that crenarchaeal homologues are divided into two groups. Group A consists of Soh PCNA A, one of the S. solfataricus PCNA homologues, and one of the Aeropyrum pernix PCNA homologues. The other crenarchaeal homologues form group B. Crenarchaeal PCNA homologues constitute a monophyletic subfamily. These results suggest that the evolution of crenarchaeal PCNA homologues has been characterized by one or two gene duplication events, which are assumed to have occurred after the split of the crenarchaeal and euryarchaeal lineages.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1431-0651
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
4
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
357-64
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| pubmed:dateRevised |
2001-11-13
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| pubmed:meshHeading |
pubmed-meshheading:11139078-Amino Acid Sequence,
pubmed-meshheading:11139078-Base Sequence,
pubmed-meshheading:11139078-Cloning, Molecular,
pubmed-meshheading:11139078-DNA Primers,
pubmed-meshheading:11139078-Molecular Sequence Data,
pubmed-meshheading:11139078-Phylogeny,
pubmed-meshheading:11139078-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:11139078-Sequence Homology, Amino Acid,
pubmed-meshheading:11139078-Sulfolobus
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| pubmed:year |
2000
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| pubmed:articleTitle |
Phylogenetic analysis of archaeal PCNA homologues.
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| pubmed:affiliation |
Department of Bioengineering, Faculty of Engineering, Soka University, Hachioji, Japan.
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| pubmed:publicationType |
Journal Article
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