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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0011065,
umls-concept:C0013935,
umls-concept:C0023810,
umls-concept:C0085862,
umls-concept:C0175697,
umls-concept:C0205263,
umls-concept:C0227525,
umls-concept:C0439851,
umls-concept:C0591833,
umls-concept:C1299583,
umls-concept:C1456820,
umls-concept:C1539220,
umls-concept:C1549571,
umls-concept:C1552596,
umls-concept:C1608386,
umls-concept:C1947931,
umls-concept:C2697656
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pubmed:issue |
4
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pubmed:dateCreated |
2000-12-27
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pubmed:abstractText |
Although it has been well known that the role of LPS on liver damage is mediated through TNF-alpha, the mechanism by which LPS modulates the cytotoxicity of IFN-gamma on hepatocytes has not yet been clearly demonstrated. Here, we demonstrate that IFN-gamma mediated apoptosis in murine embryonic hepatocyte BNL CL2 cells is potentiated by the addition of LPS (0.5 microg/ml). Consistently, LPS markedly increases the catalytic activity of caspase 3-like protease but not caspase 1-like protease in IFN-gamma treated cells. In addition, TNF-alpha alone does not affect cell viability but rather it potentiates the cytotoxic effect of IFN-gamma on BNL CL2 cells. However, the cell viability of IFN-gamma/LPS treated cells is affected by the addition of polymyxin B but not by TNF binding protein I (TNF-BPI). These data suggest that the lipid moiety of LPS may mediate direct cytotoxicity of BNL CL2 cells in a TNF-alpha independent manner.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0882-0139
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pubmed:author |
pubmed-author:ChengC YCY,
pubmed-author:ChungH THT,
pubmed-author:ColeT BTBJr,
pubmed-author:FREDE BEB,
pubmed-author:JungB HBH,
pubmed-author:KimM SMS,
pubmed-author:LamK BKB,
pubmed-author:LeeJ HJH,
pubmed-author:LiC YCY,
pubmed-author:ParkJ SJS,
pubmed-author:ParkRR,
pubmed-author:SoH SHS,
pubmed-author:VoetJJ
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
383-96
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11130781-Animals,
pubmed-meshheading:11130781-Apoptosis,
pubmed-meshheading:11130781-Caspase 3,
pubmed-meshheading:11130781-Caspases,
pubmed-meshheading:11130781-Drug Synergism,
pubmed-meshheading:11130781-Enzyme Activation,
pubmed-meshheading:11130781-Hepatocytes,
pubmed-meshheading:11130781-Interferon-gamma,
pubmed-meshheading:11130781-Lipopolysaccharides,
pubmed-meshheading:11130781-Liver,
pubmed-meshheading:11130781-Mice,
pubmed-meshheading:11130781-Recombinant Proteins,
pubmed-meshheading:11130781-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
LPS induces direct death of IFN-gamma primed murine embryonic hepatocyte, BNL CL2 cells in a TNF-alpha independent manner.
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pubmed:affiliation |
Department of Microbiology, Wonkwang University School of Medicine, Iksan, Chonbuk, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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