Linked Life Data

11124122

Source:http://linkedlifedata.com/resource/pubmed/id/11124122

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-1-26
pubmed:abstractText
The Drosophila Polycomb Group (PcG) proteins are required for stable long term transcriptional silencing of the homeotic genes. Among PcG genes, esc is unique in being critically required for establishment of PcG-mediated silencing during early embryogenesis, but not for its subsequent maintenance throughout development. We previously showed that ESC is physically associated in vivo with the PcG protein E(Z). We report here that ESC, together with E(Z), is present in a 600 kDa complex that is distinct from complexes containing other PcG proteins. We have purified this ESC complex and show that it also contains the histone deacetylase RPD3 and the histone-binding protein p55, which is also a component of the chromatin remodeling complex NURF and the chromatin assembly complex CAF-1. The association of ESC and E(Z) with p55 and RPD3 is conserved in mammals. We show that RPD3 is required for silencing mediated by a Polycomb response element (PRE) in vivo and that E(Z) and RPD3 are bound to the Ubx PRE in vivo, suggesting that they act directly at the PRE. We propose that histone deacetylation by this complex is a prerequisite for establishment of stable long-term silencing by other continuously required PcG complexes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-86
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11124122-Amino Acid Sequence, pubmed-meshheading:11124122-Animals, pubmed-meshheading:11124122-Binding Sites, pubmed-meshheading:11124122-Carrier Proteins, pubmed-meshheading:11124122-Chromosomal Proteins, Non-Histone, pubmed-meshheading:11124122-Drosophila, pubmed-meshheading:11124122-Drosophila Proteins, pubmed-meshheading:11124122-Gene Silencing, pubmed-meshheading:11124122-Genes, Homeobox, pubmed-meshheading:11124122-Genes, Insect, pubmed-meshheading:11124122-Histone Deacetylases, pubmed-meshheading:11124122-Histone-Lysine N-Methyltransferase, pubmed-meshheading:11124122-Insect Proteins, pubmed-meshheading:11124122-Macromolecular Substances, pubmed-meshheading:11124122-Mammals, pubmed-meshheading:11124122-Models, Biological, pubmed-meshheading:11124122-Molecular Sequence Data
pubmed:year
2001
pubmed:articleTitle
The Drosophila Polycomb Group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3.
pubmed:affiliation
Department of Genetics, Case Western Reserve University, Cleveland, OH 44106, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.