| pubmed-article:11120663 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0026339 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0022131 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0002520 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0017725 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0242535 | lld:lifeskim |
| pubmed-article:11120663 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:11120663 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11120663 | pubmed:dateCreated | 2001-1-12 | lld:pubmed |
| pubmed-article:11120663 | pubmed:abstractText | The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-alpha) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 +/- 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-alpha (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group (P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture (P < 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system (P < 0.05). These studies demonstrate alterations in LPS-induced TNF-alpha production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly. | lld:pubmed |
| pubmed-article:11120663 | pubmed:keyword | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11120663 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11120663 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11120663 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11120663 | pubmed:issn | 0193-1849 | lld:pubmed |
| pubmed-article:11120663 | pubmed:author | pubmed-author:SmithS MSM | lld:pubmed |
| pubmed-article:11120663 | pubmed:author | pubmed-author:SamsC FCF | lld:pubmed |
| pubmed-article:11120663 | pubmed:author | pubmed-author:TobinB WBW | lld:pubmed |
| pubmed-article:11120663 | pubmed:author | pubmed-author:Leeper-Woodfo... | lld:pubmed |
| pubmed-article:11120663 | pubmed:author | pubmed-author:HashemiB BBB | lld:pubmed |
| pubmed-article:11120663 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11120663 | pubmed:volume | 280 | lld:pubmed |
| pubmed-article:11120663 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11120663 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11120663 | pubmed:pagination | E92-102 | lld:pubmed |
| pubmed-article:11120663 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:11120663 | pubmed:meshHeading | pubmed-meshheading:11120663... | lld:pubmed |
| pubmed-article:11120663 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11120663 | pubmed:articleTitle | Altered TNF-alpha, glucose, insulin, and amino acids in islets of Langerhans cultured in a microgravity model system. | lld:pubmed |
| pubmed-article:11120663 | pubmed:affiliation | Diabetes Research Laboratory, Program in Nutrition and Biochemistry, Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, Georgia 31207, USA. tobin.b@gain.mercer.edu | lld:pubmed |
| pubmed-article:11120663 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11120663 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:11120663 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
