Source:http://linkedlifedata.com/resource/pubmed/id/11120663
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-1-12
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| pubmed:abstractText |
The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-alpha) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 +/- 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-alpha (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group (P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture (P < 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system (P < 0.05). These studies demonstrate alterations in LPS-induced TNF-alpha production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly.
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| pubmed:keyword | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Lactates,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0193-1849
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
280
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
E92-102
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11120663-Amino Acids,
pubmed-meshheading:11120663-Animals,
pubmed-meshheading:11120663-Cell Culture Techniques,
pubmed-meshheading:11120663-Cell Separation,
pubmed-meshheading:11120663-Cells, Cultured,
pubmed-meshheading:11120663-Glucose,
pubmed-meshheading:11120663-Insulin,
pubmed-meshheading:11120663-Islets of Langerhans,
pubmed-meshheading:11120663-L-Lactate Dehydrogenase,
pubmed-meshheading:11120663-Lactates,
pubmed-meshheading:11120663-Lipopolysaccharides,
pubmed-meshheading:11120663-Male,
pubmed-meshheading:11120663-Nitrogen Compounds,
pubmed-meshheading:11120663-Pancreatectomy,
pubmed-meshheading:11120663-Rats,
pubmed-meshheading:11120663-Rats, Inbred WF,
pubmed-meshheading:11120663-Tumor Necrosis Factor-alpha,
pubmed-meshheading:11120663-Weightlessness Simulation
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| pubmed:year |
2001
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| pubmed:articleTitle |
Altered TNF-alpha, glucose, insulin, and amino acids in islets of Langerhans cultured in a microgravity model system.
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| pubmed:affiliation |
Diabetes Research Laboratory, Program in Nutrition and Biochemistry, Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, Georgia 31207, USA. tobin.b@gain.mercer.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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