Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-12-26
pubmed:abstractText
Delta opioid receptors (DORs) are known to modulate multiple T-cell responses. However, little is known about the expression of these receptors. These studies evaluated the expression of DOR mRNA and protein after a single in vivo exposure to staphylococcal enterotoxin B (SEB). SEB (20 microg, ip) significantly enhanced splenocyte DOR mRNA expression 8 and 24 h after injection. SEB also increased the fractions of the total splenocyte (5 to 20%) and T-cell (8 to 50%) populations expressing DOR protein. In saline-treated animals, DOR relative fluorescence intensity per cell was 11.1 +/- 0.62 units (mean +/- SEM), increasing to 16.1 +/- 1.7 after exposure to SEB. DOR fluorescence intensity significantly increased to 33.5 +/- 2.0 units in a subpopulation of T-cells. Thus, SEB significantly increased DOR expression in vivo, affecting both mRNA and protein levels primarily within the T-cell population. To determine whether T-cell DORs modulate the activity of extracellular-regulated kinases (ERKs), the phosphorylation of ERKs 1 and 2 was studied using splenocytes from SEB-treated mice. At concentrations from 10(-8) to 10(-6) M, [d-Ala(2)-d-Leu(5)]-enkephalin, a selective DOR agonist, significantly inhibited anti-CD3-epsilon-induced phosphorylation of the ERKs. Therefore, the DORs expressed by activated T-cells are capable of attenuating T-cell activation that depends on ERK phosphorylation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0008-8749
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
205
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
84-93
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11104580-Amino Acid Sequence, pubmed-meshheading:11104580-Animals, pubmed-meshheading:11104580-Cells, Cultured, pubmed-meshheading:11104580-Enterotoxins, pubmed-meshheading:11104580-Female, pubmed-meshheading:11104580-Mice, pubmed-meshheading:11104580-Mice, Inbred BALB C, pubmed-meshheading:11104580-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:11104580-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:11104580-Mitogen-Activated Protein Kinases, pubmed-meshheading:11104580-Molecular Sequence Data, pubmed-meshheading:11104580-Phosphorylation, pubmed-meshheading:11104580-RNA, Messenger, pubmed-meshheading:11104580-Receptors, Opioid, delta, pubmed-meshheading:11104580-Spleen, pubmed-meshheading:11104580-Staphylococcus aureus, pubmed-meshheading:11104580-Superantigens
pubmed:year
2000
pubmed:articleTitle
Expression of delta opioid receptors by splenocytes from SEB-treated mice and effects on phosphorylation of MAP kinase.
pubmed:affiliation
Department of Pharmacology, University of Tennessee, Memphis, Tennessee 38163, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.