Source:http://linkedlifedata.com/resource/pubmed/id/11104580
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2000-12-26
|
pubmed:abstractText |
Delta opioid receptors (DORs) are known to modulate multiple T-cell responses. However, little is known about the expression of these receptors. These studies evaluated the expression of DOR mRNA and protein after a single in vivo exposure to staphylococcal enterotoxin B (SEB). SEB (20 microg, ip) significantly enhanced splenocyte DOR mRNA expression 8 and 24 h after injection. SEB also increased the fractions of the total splenocyte (5 to 20%) and T-cell (8 to 50%) populations expressing DOR protein. In saline-treated animals, DOR relative fluorescence intensity per cell was 11.1 +/- 0.62 units (mean +/- SEM), increasing to 16.1 +/- 1.7 after exposure to SEB. DOR fluorescence intensity significantly increased to 33.5 +/- 2.0 units in a subpopulation of T-cells. Thus, SEB significantly increased DOR expression in vivo, affecting both mRNA and protein levels primarily within the T-cell population. To determine whether T-cell DORs modulate the activity of extracellular-regulated kinases (ERKs), the phosphorylation of ERKs 1 and 2 was studied using splenocytes from SEB-treated mice. At concentrations from 10(-8) to 10(-6) M, [d-Ala(2)-d-Leu(5)]-enkephalin, a selective DOR agonist, significantly inhibited anti-CD3-epsilon-induced phosphorylation of the ERKs. Therefore, the DORs expressed by activated T-cells are capable of attenuating T-cell activation that depends on ERK phosphorylation.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/Superantigens,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin B, staphylococcal
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0008-8749
|
pubmed:author | |
pubmed:copyrightInfo |
Copyright 2000 Academic Press.
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
205
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
84-93
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:11104580-Amino Acid Sequence,
pubmed-meshheading:11104580-Animals,
pubmed-meshheading:11104580-Cells, Cultured,
pubmed-meshheading:11104580-Enterotoxins,
pubmed-meshheading:11104580-Female,
pubmed-meshheading:11104580-Mice,
pubmed-meshheading:11104580-Mice, Inbred BALB C,
pubmed-meshheading:11104580-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:11104580-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:11104580-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11104580-Molecular Sequence Data,
pubmed-meshheading:11104580-Phosphorylation,
pubmed-meshheading:11104580-RNA, Messenger,
pubmed-meshheading:11104580-Receptors, Opioid, delta,
pubmed-meshheading:11104580-Spleen,
pubmed-meshheading:11104580-Staphylococcus aureus,
pubmed-meshheading:11104580-Superantigens
|
pubmed:year |
2000
|
pubmed:articleTitle |
Expression of delta opioid receptors by splenocytes from SEB-treated mice and effects on phosphorylation of MAP kinase.
|
pubmed:affiliation |
Department of Pharmacology, University of Tennessee, Memphis, Tennessee 38163, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|