Linked Life Data

11099378

Source:http://linkedlifedata.com/resource/pubmed/id/11099378

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-1-2
pubmed:abstractText
Oct-4 is a transcription factor that is specifically expressed in mouse embryonic stem cells and in cell lines derived thereof. In these cells, Oct-4 activates transcription from remote binding sites due to as of yet unknown co-activators. Expression of Oct-4 in differentiated cells is not sufficient to activate transcription from a distance, rather it requires the co-expression of co-activators such as the adenoviral oncoprotein E1A. In this paper, we used phage display to identify Oct-4-interacting proteins. We first analyzed the interaction between Oct-4 and E1A in order to optimize the biochemical conditions that enable Oct-4-specific interactions with other interacting proteins. A panning approach was used to enrich Oct-4 interacting phages from a pool of excess unspecific phages. The biochemical conditions established in our interaction assays were then used to screen a P19 EC cell cDNA expression library in M13 filamentous phage. A number of phage clones displaying portions of unknown and known transcription factors were obtained, from which the HMG-1 transcription factor was identified. HMG-1, and the closely related factor HMG-2, interact with Oct-4 when co-expressed in mammalian cells. In addition, HMG-1 was found to cooperate with Oct-4 in P19 EC cells. These results provide the first evidence of a non-viral factor that enhances Oct-4 distance-dependent transactivation in stem cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-2836
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
304
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
529-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11099378-Adenovirus E1A Proteins, pubmed-meshheading:11099378-Animals, pubmed-meshheading:11099378-Bacteriophage M13, pubmed-meshheading:11099378-COS Cells, pubmed-meshheading:11099378-DNA-Binding Proteins, pubmed-meshheading:11099378-Germ Cells, pubmed-meshheading:11099378-High Mobility Group Proteins, pubmed-meshheading:11099378-Mice, pubmed-meshheading:11099378-Octamer Transcription Factor-3, pubmed-meshheading:11099378-Organ Specificity, pubmed-meshheading:11099378-Peptide Library, pubmed-meshheading:11099378-Precipitin Tests, pubmed-meshheading:11099378-Protein Binding, pubmed-meshheading:11099378-Recombinant Fusion Proteins, pubmed-meshheading:11099378-Transcription Factors, pubmed-meshheading:11099378-Transfection, pubmed-meshheading:11099378-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
Phage display screening reveals an association between germline-specific transcription factor Oct-4 and multiple cellular proteins.
pubmed:affiliation
Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, D-69126, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't