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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5496
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pubmed:dateCreated |
2000-11-30
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pubmed:abstractText |
beta-Arrestins, originally discovered in the context of heterotrimeric guanine nucleotide binding protein-coupled receptor (GPCR) desensitization, also function in internalization and signaling of these receptors. We identified c-Jun amino-terminal kinase 3 (JNK3) as a binding partner of beta-arrestin 2 using a yeast two-hybrid screen and by coimmunoprecipitation from mouse brain extracts or cotransfected COS-7 cells. The upstream JNK activators apoptosis signal-regulating kinase 1 (ASK1) and mitogen-activated protein kinase (MAPK) kinase 4 were also found in complex with beta-arrestin 2. Cellular transfection of beta-arrestin 2 caused cytosolic retention of JNK3 and enhanced JNK3 phosphorylation stimulated by ASK1. Moreover, stimulation of the angiotensin II type 1A receptor activated JNK3 and triggered the colocalization of beta-arrestin 2 and active JNK3 to intracellular vesicles. Thus, beta-arrestin 2 acts as a scaffold protein, which brings the spatial distribution and activity of this MAPK module under the control of a GPCR.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Arrestins,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinase 5,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/MAP2K4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MAP3K5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Map2k4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Map3k5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 10,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-arrestin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
290
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1574-7
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:11090355-Angiotensin II,
pubmed-meshheading:11090355-Animals,
pubmed-meshheading:11090355-Arrestins,
pubmed-meshheading:11090355-COS Cells,
pubmed-meshheading:11090355-Cell Line,
pubmed-meshheading:11090355-Cell Nucleus,
pubmed-meshheading:11090355-Cytosol,
pubmed-meshheading:11090355-Endosomes,
pubmed-meshheading:11090355-Enzyme Activation,
pubmed-meshheading:11090355-Humans,
pubmed-meshheading:11090355-MAP Kinase Kinase 4,
pubmed-meshheading:11090355-MAP Kinase Kinase Kinase 5,
pubmed-meshheading:11090355-MAP Kinase Kinase Kinases,
pubmed-meshheading:11090355-MAP Kinase Signaling System,
pubmed-meshheading:11090355-Mice,
pubmed-meshheading:11090355-Mitogen-Activated Protein Kinase 10,
pubmed-meshheading:11090355-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:11090355-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11090355-Mutation,
pubmed-meshheading:11090355-Phosphorylation,
pubmed-meshheading:11090355-Protein-Tyrosine Kinases,
pubmed-meshheading:11090355-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:11090355-Rats,
pubmed-meshheading:11090355-Receptor, Angiotensin, Type 1,
pubmed-meshheading:11090355-Receptors, Angiotensin,
pubmed-meshheading:11090355-Recombinant Fusion Proteins,
pubmed-meshheading:11090355-Transfection,
pubmed-meshheading:11090355-Two-Hybrid System Techniques
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pubmed:year |
2000
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pubmed:articleTitle |
Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3.
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pubmed:affiliation |
Howard Hughes Medical Institute and Department of Medicine, Duke University Medical Center, Box 3821, Durham, NC 27710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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