Linked Life Data

11090070

Source:http://linkedlifedata.com/resource/pubmed/id/11090070

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2000-12-11
pubmed:abstractText
The 5-lipoxygenase (5-LO) pathway in human CD34(+) hematopoietic progenitor cells, which were induced to differentiate into dendritic cells (DCs) by cytokines in vitro and in DCs of lymphoid tissues in situ, was examined. Extracts prepared from HPCs contained low levels of 5-LO or 5-LO-activating protein. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plus tumor necrosis factor-alpha (TNF-alpha) promoted DC differentiation and induced a strong rise in 5-LO and FLAP expression. Fluorescence-activated cell sorter (FACS) analyses identified a major DC population coexpressing human leukocyte antigen (HLA)-DR/CD80 and monocytic or Langerhans cell markers. Transforming growth factor-beta1 (TGF-beta-1), added to support DC maturation, strongly promoted the appearance of CD1a(+)/Lag(+) Langerhans-type cells as well as mature CD83(+) DCs. TGF-beta-1 further increased 5-LO and FLAP expression, recruited additional cells into the 5-LO(+) DC population, and promoted production of 5-hydroxyeicosatetraenoic acid and leukotriene B(4) in response to calcium (Ca(++)) ionophore A23187. These in vitro findings were corroborated by 5-LO expression in distinct DC phenotypes in vivo. Scattered 5-LO and FLAP in situ hybridization signals were recorded in cells of paracortical T-lymphocyte-rich areas and germinal centers (GCs) of lymph nodes (LNs) and tonsil and in cells of mucosae overlying the Waldeyer tonsillar ring. 5-LO protein localized to both CD1a(+) immature DCs and to CD83(+) mature interdigitating DCs of T-lymphocyte-rich areas of LNs and tonsil. As DCs have the unique ability to initiate naive lymphocyte activation, our data support the hypothesis that leukotrienes act at proximal steps of adaptive immune responses. (Blood. 2000;96:3857-3865)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3857-65
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11090070-Antigens, CD34, pubmed-meshheading:11090070-Arachidonate 5-Lipoxygenase, pubmed-meshheading:11090070-Arachidonic Acid, pubmed-meshheading:11090070-Calcimycin, pubmed-meshheading:11090070-Cell Differentiation, pubmed-meshheading:11090070-Cell Lineage, pubmed-meshheading:11090070-Chemotactic Factors, pubmed-meshheading:11090070-Cytokines, pubmed-meshheading:11090070-Dendritic Cells, pubmed-meshheading:11090070-Fetal Blood, pubmed-meshheading:11090070-Flow Cytometry, pubmed-meshheading:11090070-HLA-DR Antigens, pubmed-meshheading:11090070-Hematopoietic Stem Cells, pubmed-meshheading:11090070-Humans, pubmed-meshheading:11090070-Hydroxyeicosatetraenoic Acids, pubmed-meshheading:11090070-Immunohistochemistry, pubmed-meshheading:11090070-Immunophenotyping, pubmed-meshheading:11090070-Ionophores, pubmed-meshheading:11090070-Leukotriene B4, pubmed-meshheading:11090070-Lymph Nodes, pubmed-meshheading:11090070-Lymphoid Tissue, pubmed-meshheading:11090070-Macrophages, pubmed-meshheading:11090070-Palatine Tonsil
pubmed:year
2000
pubmed:articleTitle
5-lipoxygenase expression in dendritic cells generated from CD34(+) hematopoietic progenitors and in lymphoid organs.
pubmed:affiliation
Center for Vascular Medicine, Friedrich Schiller University of Jena, Erfurt, Germany. spanbroek@zmkh.ef.uni-jena.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't