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rdf:type |
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lifeskim:mentions |
umls-concept:C0006104,
umls-concept:C0017262,
umls-concept:C0034838,
umls-concept:C0036751,
umls-concept:C0079399,
umls-concept:C0086376,
umls-concept:C0178587,
umls-concept:C0185117,
umls-concept:C0205147,
umls-concept:C0206745,
umls-concept:C0301630,
umls-concept:C0596902,
umls-concept:C1415803,
umls-concept:C1705241,
umls-concept:C1705242,
umls-concept:C1948027,
umls-concept:C2911684
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pubmed:issue |
21
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pubmed:dateCreated |
2000-11-1
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pubmed:abstractText |
The aim of the present study was to investigate the mechanisms underlying the desensitization of 5-HT(1A) receptors in the dorsal raphe and hypothalamus of serotonin (5-HT) transporter knock-out mice (5-HTT -/-). The density of 5-HT(1A) receptors in the dorsal raphe was reduced in both male and female 5-HTT -/- mice. This reduction was more extensive in female than in male 5-HTT -/- mice. 8-OH-DPAT-induced hypothermia was absent in female 5-HTT -/- and markedly attenuated in 5-HTT +/- mice. The density of 5-HT(1A) receptors also was decreased significantly in several nuclei of the hypothalamus, amygdala, and septum of female 5-HTT -/- mice. 5-HT(1A) receptor mRNA was reduced significantly in the dorsal raphe region, but not in the hypothalamus or hippocampus, of female 5-HTT +/- and 5-HTT -/- mice. G-protein coupling to 5-HT(1A) receptors and G-protein levels in most brain regions were not reduced significantly, except that G(o) and G(i1) proteins were reduced modestly in the midbrain of 5-HTT -/- mice. These data suggest that the desensitization of 5-HT(1A) receptors in 5-HTT -/- mice may be attributable to a reduction in the density of 5-HT(1A) receptors. This reduction is brain region-specific and more extensive in the female mice. The reduction in the density of 5-HT(1A) receptors may be mediated partly by reduction in the gene expression of 5-HT(1A) receptors in the dorsal raphe, but also by other mechanisms in the hypothalamus of 5-HTT -/- female mice. Finally, alterations in G-protein coupling to 5-HT(1A) receptors are unlikely to be involved in the desensitization of 5-HT(1A) receptors in 5-HTT -/- mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, 5-HT1,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Slc6a4 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7888-95
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11050108-Amygdala,
pubmed-meshheading:11050108-Animals,
pubmed-meshheading:11050108-Autoradiography,
pubmed-meshheading:11050108-Binding, Competitive,
pubmed-meshheading:11050108-Carrier Proteins,
pubmed-meshheading:11050108-Female,
pubmed-meshheading:11050108-GTP-Binding Proteins,
pubmed-meshheading:11050108-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11050108-Heterozygote,
pubmed-meshheading:11050108-Hippocampus,
pubmed-meshheading:11050108-Homozygote,
pubmed-meshheading:11050108-Hypothalamus,
pubmed-meshheading:11050108-Immunoblotting,
pubmed-meshheading:11050108-Male,
pubmed-meshheading:11050108-Membrane Glycoproteins,
pubmed-meshheading:11050108-Membrane Transport Proteins,
pubmed-meshheading:11050108-Mice,
pubmed-meshheading:11050108-Mice, Knockout,
pubmed-meshheading:11050108-Nerve Tissue Proteins,
pubmed-meshheading:11050108-Organ Specificity,
pubmed-meshheading:11050108-RNA, Messenger,
pubmed-meshheading:11050108-Raphe Nuclei,
pubmed-meshheading:11050108-Receptors, Serotonin,
pubmed-meshheading:11050108-Receptors, Serotonin, 5-HT1,
pubmed-meshheading:11050108-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11050108-Septum of Brain,
pubmed-meshheading:11050108-Serotonin Antagonists,
pubmed-meshheading:11050108-Serotonin Plasma Membrane Transport Proteins,
pubmed-meshheading:11050108-Serotonin Receptor Agonists,
pubmed-meshheading:11050108-Sex Factors
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pubmed:year |
2000
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pubmed:articleTitle |
Reduction in the density and expression, but not G-protein coupling, of serotonin receptors (5-HT1A) in 5-HT transporter knock-out mice: gender and brain region differences.
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pubmed:affiliation |
Laboratory of Clinical Science, National Institute of Mental Health, National Institutes of Health Clinical Center, Bethesda, Maryland 20892-1264, USA. qianli@codon.nih.gov
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pubmed:publicationType |
Journal Article
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