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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2000-11-3
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pubmed:abstractText |
Pantetheinase (EC 3.5.1.-) is an ubiquitous enzyme which in vitro has been shown to recycle pantothenic acid (vitamin B5) and to produce cysteamine, a potent anti-oxidant. We show that the Vanin-1 gene encodes pantetheinase widely expressed in mouse tissues: (1) a pantetheinase activity is specifically expressed by Vanin-1 transfectants and is immunodepleted by specific antibodies; (2) Vanin-1 is a GPI-anchored pantetheinase, and consequently an ectoenzyme; (3) Vanin-1 null mice are deficient in membrane-bound pantetheinase activity in kidney and liver; (4) in these organs, a major metabolic consequence is the absence of detectable free cysteamine; this demonstrates that membrane-bound pantetheinase is the main source of cysteamine in tissues under physiological conditions. Since the Vanin-1 molecule was previously shown to be involved in the control of thymus reconstitution following sublethal irradiation in vivo, this raises the possibility that Vanin/pantetheinase might be involved in the regulation of some immune functions maybe in the context of the response to oxidative stress.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
483
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-54
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11042271-Amidohydrolases,
pubmed-meshheading:11042271-Animals,
pubmed-meshheading:11042271-Blotting, Northern,
pubmed-meshheading:11042271-Cell Adhesion Molecules,
pubmed-meshheading:11042271-Cell Line,
pubmed-meshheading:11042271-Cysteamine,
pubmed-meshheading:11042271-GPI-Linked Proteins,
pubmed-meshheading:11042271-Gene Expression,
pubmed-meshheading:11042271-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11042271-In Situ Hybridization,
pubmed-meshheading:11042271-Kidney,
pubmed-meshheading:11042271-Liver,
pubmed-meshheading:11042271-Membrane Proteins,
pubmed-meshheading:11042271-Mice,
pubmed-meshheading:11042271-Mice, Inbred Strains,
pubmed-meshheading:11042271-Mice, Knockout,
pubmed-meshheading:11042271-Phylogeny,
pubmed-meshheading:11042271-RNA, Messenger,
pubmed-meshheading:11042271-Tissue Distribution
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pubmed:year |
2000
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pubmed:articleTitle |
Pantetheinase activity of membrane-bound Vanin-1: lack of free cysteamine in tissues of Vanin-1 deficient mice.
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pubmed:affiliation |
Dipartmento di Biologia di Base ed Applicata Università di L'Aquila, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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