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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-1-4
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pubmed:abstractText |
TNFalpha (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression on brain microvascular endothelial cell (BMEC) culture. The tyrosine kinase (TK) inhibitor genestein (100 microgram/ml), the protein kinase C (PKC) inhibitor staurosporin (1 nM), and interferon (IF) beta-1a (1000 U/ml) antagonized TNFalpha effect. When an ineffective dose of IFbeta-1a (100 U/ml) was challenged with ineffective doses of either genestein (10 microgram/ml) or staurosporin (0.1 nM), the combination IFbeta-1a-genestein significantly reduced TNFalpha-induced ICAM1 expression whereas IFbeta-1a-staurosporin did not. These findings indicate that a TK- rather than a PKC-dependent mechanism is involved in the modulation of TNFalpha response by IFbeta-1a on BMECs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
881
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-30
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11036165-Adjuvants, Immunologic,
pubmed-meshheading:11036165-Animals,
pubmed-meshheading:11036165-Blood-Brain Barrier,
pubmed-meshheading:11036165-Cells, Cultured,
pubmed-meshheading:11036165-Down-Regulation,
pubmed-meshheading:11036165-Endothelium, Vascular,
pubmed-meshheading:11036165-Enzyme Inhibitors,
pubmed-meshheading:11036165-Genistein,
pubmed-meshheading:11036165-Humans,
pubmed-meshheading:11036165-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11036165-Interferon-beta,
pubmed-meshheading:11036165-Male,
pubmed-meshheading:11036165-Multiple Sclerosis,
pubmed-meshheading:11036165-Protein-Tyrosine Kinases,
pubmed-meshheading:11036165-Rats,
pubmed-meshheading:11036165-Rats, Wistar,
pubmed-meshheading:11036165-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
Interferon beta-1a downregulates TNFalpha-induced intercellular adhesion molecule 1 expression on brain microvascular endothelial cells through a tyrosine kinase-dependent pathway.
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pubmed:affiliation |
Department of Neurologic and Psychiatric Sciences, University of Bari, I-70124, Bari, Italy. gdefazio@neurol.uniba.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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