Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2000-11-28
pubmed:abstractText
Embryonic development in Drosophila is characterized by an early phase during which a cellular blastoderm is formed and gastrulation takes place, and by a later postgastrulation phase in which key morphogenetic processes such as segmentation and organogenesis occur. We have focused on this later phase in embryogenesis with the goal of obtaining a comprehensive analysis of the zygotic gene expression that occurs during development under normal and altered environmental conditions. For this, a functional genomic approach to embryogenesis has been developed that uses high-density oligonucleotide arrays for large-scale detection and quantification of gene expression. These oligonucleotide arrays were used for quantitative transcript imaging of embryonically expressed genes under standard conditions and in response to heat shock. In embryos raised under standard conditions, transcripts were detected for 37% of the 1,519 identified genes represented on the arrays, and highly reproducible quantification of gene expression was achieved in all cases. Analysis of differential gene expression after heat shock revealed substantial expression level changes for known heat-shock genes and identified numerous heat shock-inducible genes. These results demonstrate that high-density oligonucleotide arrays are sensitive, efficient, and quantitative instruments for the analysis of large scale gene expression in Drosophila embryos.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-10462419, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-10545926, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-10591654, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-10731132, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-2090374, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-2121748, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-2197269, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-2476281, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-2853609, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-2896587, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-6311649, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-7892198, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-8020093, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-8127661, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-8486075, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-8514184, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9117990, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9118000, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9371799, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9634850, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9649432, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9729422, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9851916, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9915496, http://linkedlifedata.com/resource/pubmed/commentcorrection/11035778-9920779
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12138-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Quantitative transcript imaging in normal and heat-shocked Drosophila embryos by using high-density oligonucleotide arrays.
pubmed:affiliation
Institute of Zoology, Biocenter/Pharmacenter, University of Basel, CH-4056 Basel, Switzerland. Ronny.Leemans@unibas.ch
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't