|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-1-17
|
|
pubmed:abstractText |
We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0962-8819
|
|
pubmed:author |
pubmed-author:ChoiI YIY,
pubmed-author:CoxR HRH,
pubmed-author:EsuM PMP,
pubmed-author:Falk,
pubmed-author:KimK HKH,
pubmed-author:KimS JSJ,
pubmed-author:KoJ HJH,
pubmed-author:LeeC SCS,
pubmed-author:LinKK,
pubmed-author:MoyF TFT,
pubmed-author:OhK BKB,
pubmed-author:PanhM HMH,
pubmed-author:ParkJ SJS,
pubmed-author:RAYM BMB,
pubmed-author:ShinS TST,
pubmed-author:YaoH LHL,
pubmed-author:YooO JOJ,
pubmed-author:YounW SWS,
pubmed-author:ZhengG DGD
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
9
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
215-22
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11032370-Animals,
pubmed-meshheading:11032370-Animals, Genetically Modified,
pubmed-meshheading:11032370-Blotting, Western,
pubmed-meshheading:11032370-Caseins,
pubmed-meshheading:11032370-Cell Division,
pubmed-meshheading:11032370-Cyclophosphamide,
pubmed-meshheading:11032370-DNA Primers,
pubmed-meshheading:11032370-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11032370-Female,
pubmed-meshheading:11032370-Genetic Vectors,
pubmed-meshheading:11032370-Goats,
pubmed-meshheading:11032370-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:11032370-Humans,
pubmed-meshheading:11032370-Mice,
pubmed-meshheading:11032370-Mice, Inbred C57BL,
pubmed-meshheading:11032370-Milk,
pubmed-meshheading:11032370-Polymerase Chain Reaction,
pubmed-meshheading:11032370-Recombinant Fusion Proteins
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat.
|
|
pubmed:affiliation |
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
