Linked Life Data

11027647

Source:http://linkedlifedata.com/resource/pubmed/id/11027647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-12-7
pubmed:abstractText
Most crystallization experiments of macromolecules are carried out at a constant temperature. Room temperature (22 degrees C) and 4 degrees C are the most widely used settings in crystallization. In practice, crystal growth at relatively high temperatures has often been avoided for macromolecular crystallization. Human estrogenic 17beta-hydroxysteroid dehydrogenase has been crystallized in complex with dehydroepiandrosterone or dihydrotestosterone. The crystallization experiments were carried out at 27 degrees C. The 17beta-HSD1 crystals were greatly improved at the elevated temperature. The effects of higher temperatures on crystal growth were studied. High temperatures stimulated the nucleation of 17beta-HSD1, increased the rate of crystal growth, and higher occupancy of substrates was obtained in the crystal structure. This method also reduced the formation of twin crystals. Since temperature is the easiest factor to control in the laboratory, crystallization at elevated temperatures provides an efficient method to improve protein crystal growth. The mechanism of the effect of temperature and relative techniques are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
100-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
The study of crystallization of estrogenic 17beta-hydroxysteroid dehydrogenase with DHEA and DHT at elevated temperature.
pubmed:affiliation
Medical Research Council Group in Molecular Endocrinology and Oncology, CHUL Research Center and Laval University, Ste-Foy, Quebec, G1V 4G2, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't