Source:http://linkedlifedata.com/resource/pubmed/id/11005752
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2000-11-6
|
| pubmed:abstractText |
Neutrophil infiltration into the pancreas is a key event in pancreatitis. Here we show that intercellular adhesion molecule-1 (ICAM-1), which regulates neutrophil adhesion, is present on rat pancreatic acinar cells, is upregulated by a hormone (cerulein) and mediates direct binding of neutrophils to acinar cells. ICAM-1 was upregulated in pancreas of rats with experimental pancreatitis induced by supramaximal doses of cerulein. Furthermore, cerulein time and dose dependently stimulated expression of ICAM-1 mRNA and protein in isolated pancreatic acinar cells. Inhibitory analysis showed that activation of transcription factor nuclear factor-kappaB (NF-kappaB) was involved in ICAM-1 upregulation by cerulein, but NF-kappaB did not mediate basal expression of ICAM-1 mRNA in acinar cells. With an adhesion assay, we found that neutrophils bind to isolated pancreatic acinar cells and that cerulein upregulates the extent of adhesion. Neutralizing ICAM-1 antibody blocked neutrophil binding to both control and cerulein-stimulated acinar cells, suggesting ICAM-1 involvement in this adhesion. Thus the acinar cell is capable of targeting neutrophils to its surface, a process that may be important for inflammatory and cell death responses in pancreatitis and other pancreatic disorders.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caerulein,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonylleucyl-leucyl-leuci...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0193-1857
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
279
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
G666-76
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11005752-Animals,
pubmed-meshheading:11005752-Caerulein,
pubmed-meshheading:11005752-Cell Adhesion,
pubmed-meshheading:11005752-Cell Nucleus,
pubmed-meshheading:11005752-Cells, Cultured,
pubmed-meshheading:11005752-Cysteine Proteinase Inhibitors,
pubmed-meshheading:11005752-Gene Expression Regulation,
pubmed-meshheading:11005752-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11005752-Kinetics,
pubmed-meshheading:11005752-Leupeptins,
pubmed-meshheading:11005752-Male,
pubmed-meshheading:11005752-Neutrophils,
pubmed-meshheading:11005752-Pancreas,
pubmed-meshheading:11005752-Pancreatitis,
pubmed-meshheading:11005752-RNA, Messenger,
pubmed-meshheading:11005752-Rats,
pubmed-meshheading:11005752-Rats, Sprague-Dawley,
pubmed-meshheading:11005752-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11005752-Transcription, Genetic
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells.
|
| pubmed:affiliation |
Department of Medicine, Veterans Affairs Greater Los Angeles Healthcare System and University of California, Los Angeles, California 90073, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|