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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
4
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pubmed:dateCreated |
2000-12-6
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pubmed:abstractText |
A procedure was developed for the preparation of anti-sulfonamide (SA) group-specific antibodies and immunosorbents. Sulfonamide haptens and conjugates were synthesized by building spacer arms on an N1 group of 4-aminobenzensulfonamide. The anti-SA group-specific antibodies and immunosorbents were prepared successfully. After extraction with methanol-water (8 + 2), sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline were cleaned up on immunoaffinity columns and determined by reversed-phase liquid chromatography with UV detection at 270 nm. The recoveries from fortified swine meat (10-100 microg/kg) ranged from 70.8 to 94.1%, with coefficients of variation of 3.4-12.9%. Limits of detection were 1-2 microg/kg.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:issn |
1060-3271
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
830-6
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pubmed:dateRevised |
2008-3-17
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pubmed:meshHeading |
pubmed-meshheading:10995109-Animals,
pubmed-meshheading:10995109-Antibody Specificity,
pubmed-meshheading:10995109-Antigens,
pubmed-meshheading:10995109-Chromatography, Affinity,
pubmed-meshheading:10995109-Haptens,
pubmed-meshheading:10995109-Immunosorbent Techniques,
pubmed-meshheading:10995109-Meat,
pubmed-meshheading:10995109-Quality Control,
pubmed-meshheading:10995109-Serum Albumin, Bovine,
pubmed-meshheading:10995109-Sulfanilamides,
pubmed-meshheading:10995109-Sulfonamides,
pubmed-meshheading:10995109-Swine
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pubmed:articleTitle |
Determination of sulfonamides in swine meat by immunoaffinity chromatography.
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|
pubmed:affiliation |
China Agricultural University, Department of Veterinary Pharmacology, Pharmacy and Toxicology, College of Veterinary Medicine, Beijing, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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