Linked Life Data

10992525

Source:http://linkedlifedata.com/resource/pubmed/id/10992525

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2000-11-3
pubmed:abstractText
The cDNA expression array technique is a powerful tool to determine, at one time from many genes, specific gene messages modulated by infection. In the present study, we identified genes modulated in response to virulent versus avirulent Legionella pneumophila infection of the alveolar macrophage cell line MH-S by the cDNA expression array technique. Many macrophage genes were found to be modulated after 5 h of in vitro infection with L. pneumophila. In particular, it was found that the monocyte chemotactic protein 3 (MCP-3) gene expression was significantly induced by infection with virulent L. pneumophila but not with avirulent L. pneumophila. In contrast, other chemokine genes, such as macrophage inflammatory protein (MIP) 1alpha, were induced by both virulent and avirulent L. pneumophila. Reverse transcription (RT)-PCR assay of total RNA isolated from macrophages infected with the bacteria for 5 or 24 h confirmed the differential induction of the chemokine genes by virulent versus avirulent L. pneumophila. Thus, the cDNA expression array technique readily revealed differential induction by L. pneumophila infection of select chemokine genes of macrophages from more than 1,100 genes. These results also indicate that certain chemokine genes may be selectively induced by virulent bacteria.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-10706689, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-2787372, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-3257460, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-6360903, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-7556453, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-7610039, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-7719907, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-8558063, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-8757834, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-9560152, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-9579370, http://linkedlifedata.com/resource/pubmed/commentcorrection/10992525-9643387
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6069-72
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Differential effects of virulent versus avirulent Legionella pneumophila on chemokine gene expression in murine alveolar macrophages determined by cDNA expression array technique.
pubmed:affiliation
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, Florida 33612, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.