Source:http://linkedlifedata.com/resource/pubmed/id/10962207
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2000-10-18
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| pubmed:abstractText |
Cat's claw (Uncaria tomentosa) is a medicinal plant from the Amazon River basin that is widely used for inflammatory disorders and was previously described as an inhibitor of NF-kappaB. Cat's claw was prepared as a decoction (water extraction) of micropulverized bark with and without concentration by freeze-drying. Murine macrophages (RAW 264.7 cells) were used in cytotoxicity assays (trypan blue exclusion) in response to the free radical 1, 1-diphenyl-2-picrilhydrazyl (DPPH, 0.3 microM) and ultraviolet light (UV) light. TNFalpha production was induced by lipopolysaccharide (LPS 0.5 microg/ml). Cat's claw was an effective scavenger of DPPH; the EC(50) value for freeze-dried concentrates was significantly less than micropulverized (18 vs. 150 microg/ml, p <.05). Cat's claw (10 microg/ml freeze-dried) was fully protective against DPPH and UV irradiation-induced cytotoxicity. LPS increased TNFalpha media levels from 3 to 97 ng/ml. Cat's claw suppressed TNFalpha production by approximately 65-85% (p <.01) but at concentrations considerably lower than its antioxidant activity: freeze-dried EC(50) = 1.2 ng/ml, micropulverized EC(50) = 28 ng/ml. In conclusion, cat's claw is an effective antioxidant, but perhaps more importantly a remarkably potent inhibitor of TNFalpha production. The primary mechanism for cat's claw anti-inflammatory actions appears to be immunomodulation via suppression of TNFalpha synthesis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2-diphenyl-1-picrylhydrazyl,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Bepridil,
http://linkedlifedata.com/resource/pubmed/chemical/Biphenyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radical Scavengers,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Picrates,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0891-5849
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
29
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
71-8
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:10962207-Animals,
pubmed-meshheading:10962207-Antioxidants,
pubmed-meshheading:10962207-Bepridil,
pubmed-meshheading:10962207-Biphenyl Compounds,
pubmed-meshheading:10962207-Cat's Claw,
pubmed-meshheading:10962207-Cell Death,
pubmed-meshheading:10962207-Cell Line,
pubmed-meshheading:10962207-Cell Survival,
pubmed-meshheading:10962207-Free Radical Scavengers,
pubmed-meshheading:10962207-Free Radicals,
pubmed-meshheading:10962207-Lipopolysaccharides,
pubmed-meshheading:10962207-Macrophages,
pubmed-meshheading:10962207-Mice,
pubmed-meshheading:10962207-Picrates,
pubmed-meshheading:10962207-Plant Extracts,
pubmed-meshheading:10962207-Plant Stems,
pubmed-meshheading:10962207-Plants, Medicinal,
pubmed-meshheading:10962207-Tumor Necrosis Factor-alpha,
pubmed-meshheading:10962207-Ultraviolet Rays
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| pubmed:year |
2000
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| pubmed:articleTitle |
Cat's claw inhibits TNFalpha production and scavenges free radicals: role in cytoprotection.
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| pubmed:affiliation |
Department of Pediatrics and Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA. sandovm@mail.amc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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