Linked Life Data

10925260

Source:http://linkedlifedata.com/resource/pubmed/id/10925260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-9-14
pubmed:abstractText
Hapten sensitization through UV-exposed skin induces hapten-specific tolerance that can be adoptively transferred by injecting T lymphocytes into naive recipients. The exact phenotype of T cells responsible for inhibiting the immune response and their mode of action remain unclear. Evidence exists that CTLA-4 negatively regulates T cell activation. We addressed whether CTLA-4 is involved in the transfer of UV-induced tolerance. Injection of lymph node cells from mice that were sensitized with dinitrofluorobenzene (DNFB) through UV-irradiated skin inhibited induction of contact hypersensitivity against DNFB in the recipient animals. When CTLA-4+ cells were depleted, transfer of suppression was lost. Likewise, significantly fewer lymphocytes enriched for CTLA-4+ cells were necessary to transfer suppression than unfractionated cells. Expression of CTLA-4 appears to be functionally relevant, since in vivo injection of a blocking anti-CTLA-4 Ab was able to break UV-induced tolerance and inhibited transfer of suppression. Upon stimulation with dendritic cells in the presence of the water-soluble DNFB analogue, DNBS, CTLA-4+ T cells from DNFB-tolerized mice secreted high levels of IL-10, TGF-beta, and IFN-gamma; low levels of IL-2; and no IL-4, resembling the cytokine pattern of T regulatory 1 cells. Ab blocking of CTLA-4 resulted in inhibition of IL-10 release. Accordingly, transfer of tolerance was not observed when recipients were treated with an anti-IL-10 Ab. Hence we propose that T cells, possibly of the T regulatory 1 type, transfer UV-mediated suppression through the release of IL-10. Activation of CTLA-4 appears to be important in this process.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1824-31
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10925260-Adoptive Transfer, pubmed-meshheading:10925260-Animals, pubmed-meshheading:10925260-Antibodies, Blocking, pubmed-meshheading:10925260-Antigens, CD, pubmed-meshheading:10925260-Antigens, Differentiation, pubmed-meshheading:10925260-CTLA-4 Antigen, pubmed-meshheading:10925260-Cells, Cultured, pubmed-meshheading:10925260-Cytokines, pubmed-meshheading:10925260-Immune Tolerance, pubmed-meshheading:10925260-Immunoconjugates, pubmed-meshheading:10925260-Immunosuppressive Agents, pubmed-meshheading:10925260-Injections, Intraperitoneal, pubmed-meshheading:10925260-Interleukin-10, pubmed-meshheading:10925260-Lymphocyte Depletion, pubmed-meshheading:10925260-Lymphocyte Transfusion, pubmed-meshheading:10925260-Mice, pubmed-meshheading:10925260-Mice, Inbred C3H, pubmed-meshheading:10925260-T-Lymphocyte Subsets, pubmed-meshheading:10925260-Ultraviolet Rays
pubmed:year
2000
pubmed:articleTitle
Evidence for functional relevance of CTLA-4 in ultraviolet-radiation-induced tolerance.
pubmed:affiliation
Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, Department of Dermatology, University of Münster, Münster, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't