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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
43
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pubmed:dateCreated |
2000-11-24
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pubmed:abstractText |
Sterol regulatory element-binding protein (SREBP)-1a is a transcription factor sensing cellular cholesterol levels and integrating gene regulatory signals mediated by MAP kinase cascades. Here we report the identification of serine 117 in SREBP-1a as the major phosphorylation site of the MAP kinases Erk1/2. This site was identified by nanoelectrospray mass spectrometry and peptide sequencing of recombinant fusion proteins phosphorylated by Erk1/2 in vitro. Serine 117 was verified as the major phosphorylation site by in vitro mutagenesis. Mutation of serine 117 to alanine abolished Erk2-mediated phosphorylation in vitro and the MAP kinase-related transcriptional activation of SREBP-1a by insulin and platelet-derived growth factor in vivo. Our data indicate that the MAP kinase-mediated effects on SREBP-1a-regulated target genes are linked to this phosphorylation site.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/SREBF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol Regulatory Element Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33302-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10915800-Amino Acid Sequence,
pubmed-meshheading:10915800-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10915800-DNA-Binding Proteins,
pubmed-meshheading:10915800-Humans,
pubmed-meshheading:10915800-Insulin,
pubmed-meshheading:10915800-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:10915800-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:10915800-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10915800-Molecular Sequence Data,
pubmed-meshheading:10915800-Phosphorylation,
pubmed-meshheading:10915800-Platelet-Derived Growth Factor,
pubmed-meshheading:10915800-Receptors, LDL,
pubmed-meshheading:10915800-Serine,
pubmed-meshheading:10915800-Sterol Regulatory Element Binding Protein 1,
pubmed-meshheading:10915800-Transcription Factors
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pubmed:year |
2000
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pubmed:articleTitle |
MAP kinases Erk1/2 phosphorylate sterol regulatory element-binding protein (SREBP)-1a at serine 117 in vitro.
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pubmed:affiliation |
Klinik II und Poliklinik für Innere Medizin am Zentrum für Molekulare Medizin der Universität zu Köln and Proteinstrukturlabor der Ruhr-Universität Bochum, 50924 Cologne, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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