Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-10-18
pubmed:abstractText
An improved method for the extraction of the major carotenoids from human milk is described. Carotenoids were extracted from milk first with ethanol and n-hexane. Then, polar xanthophylls were extracted from n-hexane into ethanol/water. The remaining n-hexane was evaporated, the residue combined with the ethanolic milk fraction and the mixture briefly saponified. Carotenoids were extracted from the hydrolysate with n-hexane, combined with the polar xanthophylls from the non-saponified ethanol/water-extract and separated by HPLC. Using this method we were able to significantly improve the recovery of xanthophylls such as lutein and zeaxanthin from human milk. The recovery rate of all carotenoids was > 90%. This method might not only be of value for milk but should be especially useful in the extraction of carotenoids from human tissues such as the adipose tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0300-9831
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
79-83
pubmed:dateRevised
2007-2-21
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Improved extraction procedure for carotenoids from human milk.
pubmed:affiliation
Institute of Nutritional Science, University of Potsdam, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't