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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-8-17
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pubmed:abstractText |
Transformed PDV keratinocytes respond to TGF-beta(1) by stimulating cell motility and invasiveness concomitantly to enhancement of the urokinase-type plasminogen activator (uPA) expression/secretion. Depletion of extracellular signal-regulated kinase (ERK1, 2) proteins by treatment of PDV cells with antisense oligonucleotides reduced basal uPA production and abolished stimulation of uPA secreted levels and cell motility by TGF-beta(1). PD098059, an inhibitor of mitogen-activated protein kinase (MAPK) kinase (MEK), decreased TGF-beta(1)-induced uPA mRNA expression, secreted activity in a dose-dependent manner, and abrogated TGF-beta(1)-stimulated cell motility and invasiveness. PDV-derived dominant-negative RasN17 cell transfectants secreted similar amounts of uPA and exhibited similar invasive abilities as the parental cells or control clones, but were unable to respond to TGF-beta(1) for stimulation of uPA-secreted levels and invasiveness. These results suggest that a Ras/MAPK transduction pathway is involved in the invasive response of transformed keratinocytes to TGF-beta(1).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
521-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10873638-Animals,
pubmed-meshheading:10873638-Base Sequence,
pubmed-meshheading:10873638-Cell Line, Transformed,
pubmed-meshheading:10873638-Cell Movement,
pubmed-meshheading:10873638-Enzyme Inhibitors,
pubmed-meshheading:10873638-Flavonoids,
pubmed-meshheading:10873638-Gene Expression,
pubmed-meshheading:10873638-Genes, ras,
pubmed-meshheading:10873638-Keratinocytes,
pubmed-meshheading:10873638-Mice,
pubmed-meshheading:10873638-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:10873638-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10873638-Mutation,
pubmed-meshheading:10873638-Oligonucleotides, Antisense,
pubmed-meshheading:10873638-Signal Transduction,
pubmed-meshheading:10873638-Transforming Growth Factor beta,
pubmed-meshheading:10873638-Urokinase-Type Plasminogen Activator,
pubmed-meshheading:10873638-ras Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Involvement of the Ras/MAPK signaling pathway in the modulation of urokinase production and cellular invasiveness by transforming growth factor-beta(1) in transformed keratinocytes.
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pubmed:affiliation |
Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, 28029-, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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