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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-7-27
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pubmed:abstractText |
Autocrine motility factor (AMF) is identical to the glycolytic enzyme phosphohexose isomerase (PHI) and overexpression of AMF/PHI is associated with tumor malignancy. In order to study the overexpression of AMF/PHI, an HA-tagged AMF construct was transiently transfected into Cos7 cells. Expression of a tagged AMF-HA allowed us to determine that over a period of 16 hours only a small amount (0.1-1%) of total cellular AMF-HA was secreted into the cell medium. Cell-associated AMF-HA was exclusively cytosolic as it could be completely extracted with Triton X-100 and concentrated within actin rich pseudopodial domains. Treatment of the cells with the glycolysis inhibitor oxamate disrupted the association of AMF-HA with actin concentrations demonstrating that glycolysis regulates the formation of these AMF/PHI-associated actin-rich protrusions. AMF/PHI is a well-characterized tumor cell secreted cytokine and we identify here an alternate intracellular function for this glycolytic enzyme/cytokine in cell motility.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
213-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10873589-Actins,
pubmed-meshheading:10873589-Animals,
pubmed-meshheading:10873589-COS Cells,
pubmed-meshheading:10873589-Cell Movement,
pubmed-meshheading:10873589-Culture Media, Conditioned,
pubmed-meshheading:10873589-Cytosol,
pubmed-meshheading:10873589-Dimerization,
pubmed-meshheading:10873589-Fluorescent Antibody Technique,
pubmed-meshheading:10873589-Gene Expression,
pubmed-meshheading:10873589-Glucose-6-Phosphate Isomerase,
pubmed-meshheading:10873589-Glycolysis,
pubmed-meshheading:10873589-L-Lactate Dehydrogenase,
pubmed-meshheading:10873589-Molecular Weight,
pubmed-meshheading:10873589-Octoxynol,
pubmed-meshheading:10873589-Oxamic Acid,
pubmed-meshheading:10873589-Protein Binding,
pubmed-meshheading:10873589-Pseudopodia,
pubmed-meshheading:10873589-Pyruvic Acid,
pubmed-meshheading:10873589-Recombinant Fusion Proteins,
pubmed-meshheading:10873589-Time Factors,
pubmed-meshheading:10873589-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
Expression of autocrine motility factor/phosphohexose isomerase in Cos7 cells.
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pubmed:affiliation |
Department of Pathology and Cell Biology, Université de Montréal, Montreal, Quebec, H3C 3J7, Canada.
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pubmed:publicationType |
Journal Article
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