Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-7-10
pubmed:abstractText
The study of lacrimal dysfunction and insufficiency, a major cause of dry eye, has been hampered by the inability to induce the proliferation of primary lacrimal acinar cells in vitro. Particularly in light of observations that androgens are able to support the overall size and functional status of the lacrimal glands as well as certain specific lacrimal functions, an in vitro culture system that is permissive for cell proliferation would be most beneficial to study the molecular basis for these processes. Here, we report on the successful establishment of such a system. Using a culture system containing Hepato Stim Medium and Matrigel, we were able to induce the efficient proliferation of primary rabbit lacrimal gland acinar cells with epidermal growth factor (EGF) and dihydrotestosterone (DHT). The generation of this in vitro cell culture system should greatly facilitate study of the regulation of acinar cell function at the molecular and cellular levels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
639-49
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10870522-Animals, pubmed-meshheading:10870522-Blotting, Western, pubmed-meshheading:10870522-Cell Division, pubmed-meshheading:10870522-Cells, Cultured, pubmed-meshheading:10870522-Collagen, pubmed-meshheading:10870522-Culture Media, pubmed-meshheading:10870522-Cyclin-Dependent Kinases, pubmed-meshheading:10870522-Drug Combinations, pubmed-meshheading:10870522-Epidermal Growth Factor, pubmed-meshheading:10870522-Extracellular Matrix, pubmed-meshheading:10870522-Female, pubmed-meshheading:10870522-Lacrimal Apparatus, pubmed-meshheading:10870522-Laminin, pubmed-meshheading:10870522-Microscopy, Electron, pubmed-meshheading:10870522-Proteoglycans, pubmed-meshheading:10870522-Rabbits, pubmed-meshheading:10870522-Stimulation, Chemical, pubmed-meshheading:10870522-Testosterone
pubmed:year
2000
pubmed:articleTitle
Proliferation of lacrimal gland acinar cells in primary culture. Stimulation by extracellular matrix, EGF, and DHT.
pubmed:affiliation
Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles 90033, USA.
pubmed:publicationType
Journal Article