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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-8-11
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pubmed:abstractText |
The vertebrate Wnt-1 proto-oncogene is expressed transiently in embryonic brain and functions in the development of the central nervous system and neural crest. The role of Wnt-1 in neural crest development appears to be to increase the number of certain progenitor cells by preventing their premature differentiation. To study the mechanism by which this transient Wnt-1 expression inhibits differentiation we have constructed PC12 pheochromocytoma cells in which Wnt-1 expression levels were controlled by use of a tetracycline-responsive transactivator. Induction of Wnt-1 expression by tetracycline withdrawal was followed by activation of the Wnt-1 signalling pathway as shown by activation of the Lef-1/Tcf transcription factor. Wnt-1 expression by these cells resulted in reversible inhibition of NGF-induced neurite outgrowth, but it did not adversely affect the maintenance of previously formed NGF-induced neurites. Wnt-1 expression also partially blocked the ability of NGF to decrease the rate of cell multiplication. Wnt-1 decreased the NGF-induced expression of the late-response gene SCG10 but not of the immediate early genes, fos, Nur77 and UPAR (urokinase-type plasminogen activator receptor) nor of the late-response genes GAP-43 and collagenase. The Wnt-1 expressing PC12 cells multiplied at a greater rate when they expressed Wnt-1 than they did in the absence of Wnt-1 expression, a result that is consistent with the proposal that Wnt-1 may also act as a mitogen.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Frizzled Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lef1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lef1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphoid Enhancer-Binding Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Plaur protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Plaur protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Urokinase Plasminogen...,
http://linkedlifedata.com/resource/pubmed/chemical/Stmn2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Stmn2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Tetracycline,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt1 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0169-328X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
232-45
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10837918-ATP-Binding Cassette Transporters,
pubmed-meshheading:10837918-Amino Acid Transport System X-AG,
pubmed-meshheading:10837918-Animals,
pubmed-meshheading:10837918-Carrier Proteins,
pubmed-meshheading:10837918-Cell Differentiation,
pubmed-meshheading:10837918-Cell Division,
pubmed-meshheading:10837918-Cell Size,
pubmed-meshheading:10837918-DNA-Binding Proteins,
pubmed-meshheading:10837918-Frizzled Receptors,
pubmed-meshheading:10837918-GAP-43 Protein,
pubmed-meshheading:10837918-Gene Expression Regulation,
pubmed-meshheading:10837918-Genes, Immediate-Early,
pubmed-meshheading:10837918-Glutamic Acid,
pubmed-meshheading:10837918-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10837918-Lymphoid Enhancer-Binding Factor 1,
pubmed-meshheading:10837918-Membrane Proteins,
pubmed-meshheading:10837918-Mice,
pubmed-meshheading:10837918-Nerve Growth Factor,
pubmed-meshheading:10837918-Nerve Growth Factors,
pubmed-meshheading:10837918-Neurites,
pubmed-meshheading:10837918-PC12 Cells,
pubmed-meshheading:10837918-Proteins,
pubmed-meshheading:10837918-Proto-Oncogene Proteins,
pubmed-meshheading:10837918-RNA, Messenger,
pubmed-meshheading:10837918-Rats,
pubmed-meshheading:10837918-Receptors, Cell Surface,
pubmed-meshheading:10837918-Receptors, Urokinase Plasminogen Activator,
pubmed-meshheading:10837918-Signal Transduction,
pubmed-meshheading:10837918-Tetracycline,
pubmed-meshheading:10837918-Transcription, Genetic,
pubmed-meshheading:10837918-Transcription Factors,
pubmed-meshheading:10837918-Tyrosine 3-Monooxygenase,
pubmed-meshheading:10837918-Wnt Proteins,
pubmed-meshheading:10837918-Wnt1 Protein,
pubmed-meshheading:10837918-Zebrafish Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Wnt-1 inhibits nerve growth factor-induced differentiation of PC12 cells by preventing the induction of some but not all late-response genes.
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pubmed:affiliation |
Department of Biological Chemistry, UCLA Medical School, University of California, 90095, Los Angeles, CA 90095, USA.
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