Source:http://linkedlifedata.com/resource/pubmed/id/10835329
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
2000-7-11
|
pubmed:abstractText |
Transforming growth factor (TGF)-beta is a family of multifunctional cytokines controlling cell growth, differentiation, and extracellular matrix deposition in the lung. The biological effects of TGF-beta are mediated by type I (TbetaR-I) and II (TbetaR-II) receptors. Our previous studies show that the expression of TbetaR-II is highly regulated in a spatial and temporal fashion during lung development. In the present studies, we investigated the temporal-spatial pattern and cellular expression of TbetaR-I during lung development. The expression level of TbetaR-I mRNA in rat lung at different embryonic and postnatal stages was analyzed by Northern blotting. TbetaR-I mRNA was expressed in fetal rat lungs in early development and then decreased as development proceeded. The localization of TbetaR-I in fetal and postnatal rat lung tissues was investigated by using in situ hybridization performed with an antisense RNA probe. TbetaR-I mRNA was present in the mesenchyme and epithelium of gestational day 14 rat lungs. An intense TbetaR-I signal was observed in the epithelial lining of the developing bronchi. In gestational day 16 lungs, the expression of TbetaR-I mRNA was increased in the mesenchymal tissue. The epithelium in both the distal and proximal bronchioles showed a similar level of TbetaR-I expression. In postnatal lungs, TbetaR-I mRNA was detected in parenchymal tissues and blood vessels. We further studied the expression of TbetaR-I in cultured rat lung cells. TbetaR-I was expressed by cultured rat lung fibroblasts, microvascular endothelial cells, and alveolar epithelial cells. These studies demonstrate a differential regulation and localization of TbetaR-I that is different from that of TbetaR-II during lung development. TbetaR-I, TbetaR-II, and TGF-beta isoforms exhibit distinct but overlapping patterns of expression during lung development. This implies a distinct role for TbetaR-I in mediating TGF-beta signal transduction during lung development.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activin Receptors, Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/TGF-beta type I receptor
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
1040-0605
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
278
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
L1231-9
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:10835329-Activin Receptors, Type I,
pubmed-meshheading:10835329-Aging,
pubmed-meshheading:10835329-Animals,
pubmed-meshheading:10835329-Animals, Newborn,
pubmed-meshheading:10835329-Blotting, Northern,
pubmed-meshheading:10835329-Cells, Cultured,
pubmed-meshheading:10835329-Embryonic and Fetal Development,
pubmed-meshheading:10835329-Fetus,
pubmed-meshheading:10835329-In Situ Hybridization,
pubmed-meshheading:10835329-Lung,
pubmed-meshheading:10835329-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10835329-RNA, Messenger,
pubmed-meshheading:10835329-Rats,
pubmed-meshheading:10835329-Rats, Sprague-Dawley,
pubmed-meshheading:10835329-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:10835329-Tissue Distribution
|
pubmed:year |
2000
|
pubmed:articleTitle |
Ontogeny and localization of TGF-beta type I receptor expression during lung development.
|
pubmed:affiliation |
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA. zhaoyun@duke.edu
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|