Linked Life Data

10832591

Source:http://linkedlifedata.com/resource/pubmed/id/10832591

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-9-21
pubmed:abstractText
Apoptosis is associated with caspase-mediated proteolysis of Type I (K18 and K19) cytokeratins. We previously showed a positive association between the levels of tissue polypeptide antigen (TPA), that recognizes cytokeratins K8, K18, and K19 fragments, and induced apoptosis in breast cancer cell lines. The aim of the present study was to evaluate the interrelationships between TPA, steroid receptors, and p53, and their joint prognostic role in node-negative breast cancer patients not treated with adjuvant therapies. Age and pT were also considered since they are known prognostic factors. Five hundred and ninety-nine cases with N- breast cancer were evaluated (median follow-up: 60 months). TPA was measured by an immunoradiometric assay and p53 by an immunochemiluminescent assay in tumor cytosol. Multiple correspondence analysis was used to study the associations among variables. Their prognostic role (univariate analysis) and their joint effect (multivariate analysis) on RFS were investigated with Cox regression models. TPA showed a direct association with ER and PgR. Higher p53 values were weakly associated to low values of ER, PgR, and TPA. Younger age was related to low and intermediate values of ER and PgR and to low p53 values, while older age was related to high values of ER. Multivariate analysis showed a significant prognostic impact for pT, age, ER, and TPA. Among the interactions considered clinically relevant, only that between ER and age was found. RFS estimated values were poorer in cases with lower than in those with higher TPA values, both in patients expected to have a poor (pT2, young age, low ER) and a better prognosis (pT1, older age, high ER). From the findings of the present study we can draw the following conclusions: The relationship of TPA with prognosis gives an additional contribution to pT, age, and steroid receptors in N- breast cancer; TPA may be considered the first marker of apoptosis measured with a fully standardized quantitative method in tumor cytosol and could be evaluated in prognostic indexes including markers related to different biological mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0167-6806
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10832591-Adult, pubmed-meshheading:10832591-Age Factors, pubmed-meshheading:10832591-Aged, pubmed-meshheading:10832591-Aged, 80 and over, pubmed-meshheading:10832591-Apoptosis, pubmed-meshheading:10832591-Breast Neoplasms, pubmed-meshheading:10832591-Cytosol, pubmed-meshheading:10832591-Disease-Free Survival, pubmed-meshheading:10832591-Female, pubmed-meshheading:10832591-Humans, pubmed-meshheading:10832591-Italy, pubmed-meshheading:10832591-Keratins, pubmed-meshheading:10832591-Middle Aged, pubmed-meshheading:10832591-Prognosis, pubmed-meshheading:10832591-Receptors, Estrogen, pubmed-meshheading:10832591-Receptors, Progesterone, pubmed-meshheading:10832591-Solubility, pubmed-meshheading:10832591-Tissue Polypeptide Antigen, pubmed-meshheading:10832591-Tumor Markers, Biological, pubmed-meshheading:10832591-Tumor Suppressor Protein p53
pubmed:year
2000
pubmed:articleTitle
Quantitative measurement of soluble cytokeratin fragments in tissue cytosol of 599 node negative breast cancer patients: a prognostic marker possibly associated with apoptosis.
pubmed:affiliation
Centro Regionale per lo Studio degli Indicatori Biochimici di Tumore, Ospedale Civile, Venezia, Italy. cnabo@provincia.venezia.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't