Linked Life Data

10831134

Source:http://linkedlifedata.com/resource/pubmed/id/10831134

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-8-28
pubmed:abstractText
Phage display comprises an array of methods, which can be used to display proteins on bacteriophages. We present in this article a summary of techniques, which can be used to express antibody libraries on bacteriophages. Since many researchers are more familiar with the conventional hybridoma technique for production of monoclonal antibodies we describe analogies and differences between these two techniques, both of which are used to reach comparable scientific objectives. We focus on the features which are specific to phage display techniques rather than for hybridoma techniques. These comprise the freedom to choose other animals than the mouse for immunization, the enormously large sample (up to 10(9) clones) which can be drawn and immortalized from a single immunized animal and the opportunity to enhance affinity of isolated antibodies by in vitro affinity maturation. The panning techniques, which are used to enrich specifically binding phage antibodies from the huge libraries are briefly summarized.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-4004
pubmed:author
pubmed:issnType
Print
pubmed:volume
21 Suppl A
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S106-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Phage display: a molecular tool for the generation of antibodies--a review.
pubmed:affiliation
Department of Anatomy, University of Technology, Aachen, Germany. ulrike.schmitz@post.rwth-aachen.de
pubmed:publicationType
Journal Article, Comparative Study, Review