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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2000-6-7
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pubmed:abstractText |
We investigated the capacity of mouse Langerhans cells (LC) to produce IL-12, a central cytokine in a Th1 type of immune responses. We prepared purified LC (>95%) from BALB/c mouse skin by the panning method using anti-I-Ad mAb. An ELISA showed that purified LC spontaneously produced IL-12 p40, and that its production was up-regulated following simultaneous stimulation with anti-CD40 mAb and IFN-gamma. Surprisingly, GM-CSF strikingly inhibited IL-12 p40 production by anti-CD40/IFN-gamma-stimulated LC (% inhibition = 97.0 +/- 0.9% at 1 ng/ml GM-CSF). Supernatants of 48-h cultured keratinocytes (KC) also caused the inhibition of LC IL-12 p40 secretion, and this effect was neutralized by anti-GM-CSF mAb. IL-1alpha (1 ng/ml)-stimulated KC produced much more GM-CSF than unstimulated KC (60.9 +/- 0.2 pg/ml vs 20.9 +/- 1.7 pg/ml), and IL-1alpha-stimulated KC supernatants strongly inhibited IL-12 p40 production by anti-CD40/IFN-gamma-stimulated LC (% inhibition = 89.4 +/- 1.4%). A bioassay using an IL-12-dependent T cell line demonstrated the correlation of the level of IL-12 p40 with the bioactivity of IL-12. These results provide important implications for the pathogenesis of atopic dermatitis, which involves the participation of LC and KC with the capacity to produce IL-12 and GM-CSF, respectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5113-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10799868-Animals,
pubmed-meshheading:10799868-Antibodies, Monoclonal,
pubmed-meshheading:10799868-Antigens, CD,
pubmed-meshheading:10799868-Antigens, CD40,
pubmed-meshheading:10799868-Cell Separation,
pubmed-meshheading:10799868-Cells, Cultured,
pubmed-meshheading:10799868-Dendritic Cells,
pubmed-meshheading:10799868-Down-Regulation,
pubmed-meshheading:10799868-Female,
pubmed-meshheading:10799868-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:10799868-Immunosuppressive Agents,
pubmed-meshheading:10799868-Interferon-gamma,
pubmed-meshheading:10799868-Interleukin-12,
pubmed-meshheading:10799868-Keratinocytes,
pubmed-meshheading:10799868-Langerhans Cells,
pubmed-meshheading:10799868-Membrane Glycoproteins,
pubmed-meshheading:10799868-Mice,
pubmed-meshheading:10799868-Mice, Inbred BALB C,
pubmed-meshheading:10799868-Spleen,
pubmed-meshheading:10799868-Up-Regulation
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pubmed:year |
2000
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pubmed:articleTitle |
Granulocyte/macrophage colony-stimulating factor inhibits IL-12 production of mouse Langerhans cells.
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pubmed:affiliation |
Department of Dermatology, University of Tokyo, Tokyo, Japan. ytada-tky@umin.ac.jp
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pubmed:publicationType |
Journal Article
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